Tunable biomolecular interaction and fluorescence quenching ability of graphene oxide: application to "turn-on" DNA sensing in biological media.

As a platform for "turn-on" DNA sensing, the level of oxidation of graphene oxide strongly affects its fluorescence quenching ability and binding interactions to single-stranded oligodeoxyribonucleotides (ssODNs), leading to a broad range of sensitivity. Fine-tuning the level of oxidation of graphene oxide yields a DNA-detection platform that is highly sensitive in serum and biological media.

[1]  Sandip Niyogi,et al.  Solution properties of graphite and graphene. , 2006, Journal of the American Chemical Society.

[2]  Xi Chen,et al.  Graphene oxide-protected DNA probes for multiplex microRNA analysis in complex biological samples based on a cyclic enzymatic amplification method. , 2012, Chemical communications.

[3]  Bong Jin Hong,et al.  Nanoscale-controlled spacing provides DNA microarrays with the SNP discrimination efficiency in solution phase. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[4]  H. Rothuizen,et al.  Translating biomolecular recognition into nanomechanics. , 2000, Science.

[5]  D R Walt,et al.  Biological warfare detection. , 2000, Analytical chemistry.

[6]  Ronghua Yang,et al.  Carbon nanotube-quenched fluorescent oligonucleotides: probes that fluoresce upon hybridization. , 2008, Journal of the American Chemical Society.

[7]  Candace R. Spier,et al.  Near real‐time, on‐site, quantitative analysis of PAHs in the aqueous environment using an antibody‐based biosensor , 2011, Environmental toxicology and chemistry.

[8]  Huang-Hao Yang,et al.  Increasing the sensitivity and single-base mismatch selectivity of the molecular beacon using graphene oxide as the "nanoquencher". , 2010, Chemistry.

[9]  Yuehe Lin,et al.  Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells. , 2010, Journal of the American Chemical Society.

[10]  Huixiang Li,et al.  Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Jacek Klinowski,et al.  Structure of Graphite Oxide Revisited , 1998 .

[12]  M. Pumera,et al.  Impedimetric thrombin aptasensor based on chemically modified graphenes. , 2012, Nanoscale.

[13]  H. S. Wolff,et al.  iRun: Horizontal and Vertical Shape of a Region-Based Graph Compression , 2022, Sensors.

[14]  S. Nguyen,et al.  Graphene oxide, highly reduced graphene oxide, and graphene: versatile building blocks for carbon-based materials. , 2010, Small.

[15]  R. Lequin.  Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). , 2005, Clinical chemistry.

[16]  Leif O. Brown,et al.  Reversible fluorescence quenching in carbon nanotubes for biomolecular sensing. , 2007, Nature nanotechnology.

[17]  Ronghua Yang,et al.  Noncovalent assembly of carbon nanotubes and single-stranded DNA: an effective sensing platform for probing biomolecular interactions. , 2008, Analytical chemistry.

[18]  J. D. Lopez-Gonzalez,et al.  Study of oxygen-containing groups in a series of graphite oxides: Physical and chemical characterization , 1995 .

[19]  Hongje Jang,et al.  A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase† , 2010, Angewandte Chemie.

[20]  S. Stankovich,et al.  Synthesis and exfoliation of isocyanate-treated graphene oxide nanoplatelets , 2006 .

[21]  Robert M. Corn,et al.  Ultrasensitive DNA microarray biosensing via in situ RNA transcription-based amplification and nanoparticle-enhanced SPR imaging. , 2011, Journal of the American Chemical Society.

[22]  Chun Li,et al.  Chemically converted graphene induced molecular flattening of 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin and its application for optical detection of cadmium(II) ions. , 2009, Journal of the American Chemical Society.

[23]  Bong Jin Hong,et al.  DNA microarrays on nanoscale-controlled surface , 2005, Nucleic acids research.

[24]  Wei Tao Huang,et al.  A reversible fluorescence nanoswitch based on bifunctional reduced graphene oxide: use for detection of Hg2+ and molecular logic gate operation. , 2011, Chemical communications.

[25]  Chunhai Fan,et al.  A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis , 2010 .

[26]  K. L. Sebastian,et al.  Long range resonance energy transfer from a dye molecule to graphene has (distance)(-4) dependence. , 2009, The Journal of chemical physics.

[27]  Rolf Erni,et al.  Determination of the Local Chemical Structure of Graphene Oxide and Reduced Graphene Oxide , 2010, Advanced materials.

[28]  Huajian Gao,et al.  SIMULATION OF DNA-NANOTUBE INTERACTIONS , 2004 .

[29]  M. Heller.  DNA microarray technology: devices, systems, and applications. , 2002, Annual review of biomedical engineering.

[30]  Xiangqun Zeng,et al.  Nonlabeled quartz crystal microbalance biosensor for bacterial detection using carbohydrate and lectin recognitions. , 2007, Analytical chemistry.

[31]  R. Stoltenberg,et al.  Evaluation of solution-processed reduced graphene oxide films as transparent conductors. , 2008, ACS nano.

[32]  G. Wallace,et al.  Processable aqueous dispersions of graphene nanosheets. , 2008, Nature nanotechnology.

[33]  Imre Dékány,et al.  Evolution of surface functional groups in a series of progressively oxidized graphite oxides , 2006 .

[34]  Bong Jin Hong,et al.  Successful stabilization of graphene oxide in electrolyte solutions: enhancement of biofunctionalization and cellular uptake. , 2012, ACS nano.

[35]  Franklin Kim,et al.  Langmuir-Blodgett assembly of graphite oxide single layers. , 2009, Journal of the American Chemical Society.

[36]  Zhuang Liu,et al.  PEGylated nanographene oxide for delivery of water-insoluble cancer drugs. , 2008, Journal of the American Chemical Society.

[37]  Martin Pumera,et al.  Graphene materials preparation methods have dramatic influence upon their capacitance , 2012 .

[38]  Matthew A. Cooper,et al.  Optical biosensors in drug discovery , 2002, Nature Reviews Drug Discovery.

[39]  Sanjay Tyagi,et al.  Molecular Beacons: Probes that Fluoresce upon Hybridization , 1996, Nature Biotechnology.

[40]  Jiali Zhang,et al.  Biocompatibility of Graphene Oxide , 2010, Nanoscale research letters.

[41]  R. Mcglennen,et al.  Miniaturization technologies for molecular diagnostics. , 2001, Clinical chemistry.

[42]  Daniel A. Zweifel,et al.  Interactions of functionalized carbon nanotubes with tethered pyrenes in solution , 2002 .

[43]  Emanuel F Petricoin,et al.  Protein microarray detection strategies: focus on direct detection technologies. , 2004, Journal of immunological methods.

[44]  K. L. Sebastian,et al.  Resonance energy transfer from a dye molecule to graphene. , 2008, The Journal of chemical physics.

[45]  Ronald W. Davis,et al.  Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray , 1995, Science.

[46]  M. Melucci,et al.  High-contrast visualization of graphene oxide on dye-sensitized glass, quartz, and silicon by fluorescence quenching. , 2009, Journal of the American Chemical Society.

[47]  Dongmin Chen,et al.  Synthesis and Solid-State NMR Structural Characterization of 13C-Labeled Graphite Oxide , 2008, Science.

[48]  Huang-Hao Yang,et al.  A graphene platform for sensing biomolecules. , 2009, Angewandte Chemie.

[49]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[50]  G. Eda,et al.  Chemically Derived Graphene Oxide: Towards Large‐Area Thin‐Film Electronics and Optoelectronics , 2010, Advanced materials.

[51]  Visualizing graphene based sheets by fluorescence quenching microscopy. , 2009, Journal of the American Chemical Society.

[52]  Jian-hui Jiang,et al.  Graphene fluorescence resonance energy transfer aptasensor for the thrombin detection. , 2010, Analytical chemistry.

[53]  R. Tecon,et al.  Bacterial Biosensors for Measuring Availability of Environmental Pollutants , 2008, Sensors.

[54]  M. Pumera,et al.  Electrochemistry at chemically modified graphenes. , 2011, Chemistry.