'Flavescence dorée' phytoplasma genome: a metabolism oriented towards glycolysis and protein degradation.

The 670 kbp chromosome of 'flavescence doree' phytoplasma line FD92 was partially sequenced by pyrosequencing and SOLEXA. More than 94% of the chromosome could be assembled and the 22 largest contigs representing 85% of the chromo- some were annotated under the semi-automatic annotation platform iANT. Out of 464 chromosomal coding sequences (CDS), 174 CDS (38%) were involved in information transfer (DNA replication, protein production, RNA modification and regulation), 88 CDS (19%) were encoding metabolic enzymes, 40 CDS (9%) corresponded to transporters, 8 CDS (1%) corresponded to cellular processes, whereas 145 CDS (31%) remained cryptic. At this stage of incomplete assembly, repeated sequences were underesti- mated and transposon and phage-related CDS (2%) could not yet be precisely evaluated. FD92 phytoplasma possesses a complete glycolytic pathway and has a prominent system for proteolysis possibly resulting from the adaptation to its woody hosts.