Expression profiles of two thaumatin‐like protein (TmTLP) genes in responses to various micro‐organisms from Tenebrio molitor

It is well known that anti‐fungal thaumatin‐like proteins (TLPs) play important roles in plants. Here, we investigated the expression analysis of thaumatin‐like protein genes TmTLPs in response to various pathogens in Tenebrio molitor. Developmental expression patterns of TmTLPs show that TmTLPs are highly expressed in the early pupal and adult stages. Furthermore, tissue‐specific expression patterns of TmTLPs indicate that TmTLP1 is highly expressed in the integument and gut of last instar larvae and the integument and Malpighian tubules of 5‐day old adults. In contrast, TmTLP2 is highly expressed in the gut of both last instar larvae and 5‐day old adults. We hypothesize that the expression of TmTLP genes in developmental stages may be related to molting and body remodeling stresses. In addition, the induction patterns of TmTLP genes indicate that TmTLPs were slightly induced by Escherichia coli, Staphylococcus aureus, Candida albicans and Listeria monocytogenes. Furthermore, TmTLP1 and TmTLP2 were strongly induced in response to E. coli at 9 h post‐injection and L. monocytogenes at 3 h post‐injection. Our results suggest that TmTLPs may possess antimicrobial functions in T. molitor.

[1]  Mario Riera Romo,et al.  Innate immunity in vertebrates: an overview , 2016, Immunology.

[2]  Y. Han,et al.  Cloning, expression analysis, and RNA interference study of a HORMA domain containing autophagy-related gene 13 (ATG13) from the coleopteran beetle, Tenebrio molitor , 2015, Front. Physiol..

[3]  P. Kirti,et al.  Characterization of a Pathogen Induced Thaumatin-Like Protein Gene AdTLP from Arachis diogoi, a Wild Peanut , 2013, PloS one.

[4]  J. Dean,et al.  Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions. , 2012, Tree physiology.

[5]  Fuguang Li,et al.  Purification and Characterization of a CkTLP Protein from Cynanchum komarovii Seeds that Confers Antifungal Activity , 2011, PloS one.

[6]  Lili Huang,et al.  Characterization of a pathogenesis-related thaumatin-like protein gene TaPR5 from wheat induced by stripe rust fungus. , 2010, Physiologia plantarum.

[7]  Seung‐Won Park,et al.  Characterization and cDNA cloning of a cecropin-like antimicrobial peptide, papiliocin, from the swallowtail butterfly, Papilio xuthus , 2010, Molecules and cells.

[8]  Yinghua Huang,et al.  Differential Expression of Thaumatin-Like Proteins in Sorghum Infested with Greenbugs , 2010, Zeitschrift fur Naturforschung. C, Journal of biosciences.

[9]  V. Deretic,et al.  Autophagy, immunity, and microbial adaptations. , 2009, Cell host & microbe.

[10]  M. Belosevic,et al.  Hymenolepis diminuta (Cestoda) induces changes in expression of select genes of Tribolium confusum (Coleoptera) , 2009, Parasitology Research.

[11]  D. Hultmark,et al.  Comparative genomic analysis of the Tribolium immune system , 2007, Genome Biology.

[12]  L. Pucillo,et al.  Purification and characterization of an antifungal thaumatin-like protein from Cassia didymobotrya cell culture. , 2006, Plant physiology and biochemistry : PPB.

[13]  J. Duman,et al.  A thaumatin-like protein from larvae of the beetle Dendroides canadensis enhances the activity of antifreeze proteins. , 2006, Biochemistry.

[14]  C. Cambillau,et al.  Plant stress proteins of the thaumatin‐like family discovered in animals , 2004, FEBS Letters.

[15]  K. Mita,et al.  Characterization and transcriptional profiles of three Spodoptera frugiperda genes encoding cysteine-rich peptides. A new class of defensin-like genes from lepidopteran insects? , 2003, Gene.

[16]  A. Vilcinskas,et al.  Cloning and expression of gallerimycin, an antifungal peptide expressed in immune response of greater wax moth larvae, Galleria mellonella. , 2003, Archives of insect biochemistry and physiology.

[17]  N. Campillo,et al.  Plant-based heterologous expression of Mal d 2, a thaumatin-like protein and allergen of apple (Malus domestica), and its characterization as an antifungal protein. , 2003, Journal of molecular biology.

[18]  Dong-ha Oh,et al.  Isolation of a cDNA Encoding a 31-kDa, Pathogenesis-related 5/thaumatin-like (PR5/TL) Protein Abundantly Expressed in Apple Fruit (Malus domestica cv. Fuji) , 2000, Bioscience, biotechnology, and biochemistry.

[19]  T. Ng,et al.  First chromatographic isolation of an antifungal thaumatin-like protein from French bean legumes and demonstration of its antifungal activity. , 1999, Biochemical and biophysical research communications.

[20]  Byong-Seok Choi,et al.  Structural characteristics of tenecin 3, an insect antifungal protein , 1999, Biochemistry and molecular biology international.

[21]  J. Chung,et al.  Bacterial expression of tenecin 3, an insect antifungal protein isolated from Tenebrio molitor, and its efficient purification. , 1998, Molecules and Cells.

[22]  M. Ptak,et al.  Solution structure of drosomycin, the first inducible antifungal protein from insects , 1997, Protein science : a publication of the Protein Society.

[23]  A. Reddy,et al.  Cloning and expression of a PR5-like protein from Arabidopsis: inhibition of fungal growth by bacterially expressed protein , 1997, Plant Molecular Biology.

[24]  A. van Dorsselaer,et al.  A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution. , 1993, The Journal of biological chemistry.

[25]  D. Hultmark,et al.  Insect immunity. Characterization of a Drosophila cDNA encoding a novel member of the diptericin family of immune peptides. , 1990, The Journal of biological chemistry.

[26]  J. Imler.  Overview of Drosophila immunity: a historical perspective. , 2014, Developmental and comparative immunology.

[27]  B. Altincicek,et al.  Beetle immunity: Identification of immune-inducible genes from the model insect Tribolium castaneum. , 2008, Developmental and comparative immunology.

[28]  P. Rougé,et al.  A molecular basis for the endo-beta 1,3-glucanase activity of the thaumatin-like proteins from edible fruits. , 2003, Biochimie.

[29]  M. Kanost,et al.  Biological mediators of insect immunity. , 1997, Annual review of entomology.

[30]  M. Strand,et al.  Immunological basis for compatibility in parasitoid-host relationships. , 1995, Annual review of entomology.

[31]  Jae Eun Lee,et al.  Glycogen Synthase Kinase-3 Interaction Domain Enhances Phosphorylation of SARS-CoV-2 Nucleocapsid Protein , 2013, Molecules and cells.