Restriction enzyme analysis and ribotyping distinguish Bordetella avium and Bordetella hinzii isolates

Fifty-seven bacterial isolates previously identified as Bordetella avium or B. hinzii were characterized by restriction enzyme analysis (REA) and/or ribotyping. Twenty restriction endonucleases were evaluated for REA. Digestion of chromosomal DNA from the 42 B. avium and 15 B. hinzii isolates with Hinf I produced 8 and 7 distinct fingerprint profiles, respectively. Digestion with DdeI further discriminated these Bordetella species and produced 12 fingerprint profiles for B. avium and 4 profiles of B. hinzii. In addition, B. avium isolates were clearly distinguishable from B. hinzii isolates by ribotyping with the restriction endonuclease PvuII. The ribotype patterns of these two species of Bordetella were unique when compared to previously reported ribotype patterns for B. bronchiseptica isolates. Since it was possible to discern differences among isolates within each Bordetella species by REA analysis, we suggest that REA could be used in developing a typing system based on the fingerprint profiles generated.

[1]  R. Rimler,et al.  DNA fingerprinting of Riemerella anatipestifer. , 1998, Avian diseases.

[2]  M. Ackermann,et al.  Use of ribotyping to distinguish Bordetella bronchiseptica isolates. , 1997, International journal of systematic bacteriology.

[3]  P. de Vos,et al.  Bordetella trematum sp. nov., isolated from wounds and ear infections in humans, and reassessment of Alcaligenes denitrificans Rüger and Tan 1983. , 1996, International journal of systematic bacteriology.

[4]  P. Vandamme,et al.  Characteristics of Bordetella hinzii strains isolated from a cystic fibrosis patient over a 3-year period , 1996, Journal of clinical microbiology.

[5]  P. Vandamme,et al.  Differentiation of Bordetella-pertussis, B-parapertussis, and B-bronchiseptica by whole-cell protein electrophoresis and fatty-acid analysis. , 1995 .

[6]  P. Vandamme,et al.  Bordetella hinzii sp. nov., isolated from poultry and humans. , 1995, International journal of systematic bacteriology.

[7]  C. W. Moss,et al.  Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia , 1995, Journal of clinical microbiology.

[8]  I. Gardner,et al.  Molecular Fingerprinting of Pasteurella Multocida Associated with Progressive Atrophic Rhinitis in Swine Herds , 1994, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[9]  J. Harel,et al.  Characterization of Serpulina hyodysenteriae isolates of serotypes 8 and 9 from Quebec by restriction endonuclease fingerprinting and ribotyping. , 1994, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.

[10]  P. Vandamme,et al.  Bacteremia caused by a novel Bordetella species, "B. hinzii" , 1994, Journal of clinical microbiology.

[11]  T. Stull,et al.  A subcloned ribosomal RNA probe for bacterial ribotype analysis. , 1994, The Journal of infectious diseases.

[12]  R. Matthews,et al.  Genetic relatedness of Bordetella species as determined by macrorestriction digests resolved by pulsed-field gel electrophoresis. , 1993, International journal of systematic bacteriology.

[13]  M. A. Wilson,et al.  Comparison of DNA fingerprints and somatic serotypes of serogroup B and E Pasteurella multocida isolates , 1992, Journal of clinical microbiology.

[14]  C. J. Moore,et al.  Differentiation of Bordetella avium and related species by cellular fatty acid analysis , 1987, Journal of clinical microbiology.

[15]  Y. Saif,et al.  Contribution to the taxonomy of the turkey coryza agent: cellular fatty acid analysis of the bacterium. , 1986, Avian diseases.

[16]  Y. Saif,et al.  Further characterization of the agent causing coryza in turkeys. , 1985, Avian diseases.

[17]  Rimler Rb,et al.  Differentiation Among Bacteria Isolated from Turkeys with Coryza (Rhinotracheitis) , 1983 .

[18]  D. G. Simmons,et al.  Differentiation among bacteria isolated from turkeys with coryza (rhinotracheitis). , 1983, Avian diseases.

[19]  M. M. Jensen,et al.  Immunization against turkey coryza by colonization with mutants of Alcaligenes faecalis. , 1980, Avian diseases.