The use of spectral fluorescence methods to detect changes in the phytoplankton community

In vivo fluorescence methods are efficient toolsfor studying the seasonal and spatial dynamics ofphytoplankton. Traditionally the measurements are madeusing single excitation-emission wavelengthcombination. During a cruise in the Gulf of Riga(Baltic Sea) we supplemented this technique bymeasuring the spectral fluorescence signal (SFS) andfixed wavelength fluorescence intensities at theexcitation maxima of main accessory pigments. Thesemethods allowed the rapid collection of quantitativefluorescence data and chemotaxonomic diagnostics ofthe phytoplankton community. The chlorophylla-specific fluorescence intensities (R) and thespectral fluorescence fingerprints were analysedtogether with concentrations of chlorophyll a indifferent algal size-groups, phytoplankton biomass andtaxonomic position. The lower level of R in thesouthern gulf was related to the higher proportion ofcyanobacteria relative to total biomass and the lowerabundance of small algae. The phycoerythrinfluorescence signal was obviously due to the largecyanobacteria. The basin-wide shift in the shape ofchlorophyll a excitation spectra was caused bythe variable proportions of differently pigmentedcyanobacteria, diatoms and cryptomonads.

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