Genetic Diversity Assessment In Deep Water Rice Found In Manipur, India Using Molecular Markers

Molecular markers were used to study genetic diversity among the eight selected rice genotypes where six are local genotypes found in Manipur. A total of 19 RAPD, 17 ISSR and 29 SSR primers were screened where 13 RAPD, 12 ISSR and 20 SSR generated a total of 90, 88 and 160 alleles were detected respectively. The average number of alleles per locus was 6.9, 7.3 and 8.0 for each marker. The polymorphic information content (PIC) value ranged from 0.38 to 0.86 in RAPD, 0.16 to 0.79 in ISSR and 0.54 to 0.88 with SSR analysis. A combined cluster analysis was performed using UPGMA using the DICE’s similarity coefficient and the resulting dendrogram resolved the selected rice cultivars into two major clusters value ranging from 0.66 to 0.79. From the results, it is proven that this study can be useful in polymorphism analysis and genetic improvements by breeding in future.

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