PLASMODESMAL MODIFICATIONS AND DISTRIBUTION OF TOSPO VIRUS IN POTATO AND TOMATO

ABSTRACT The tospovirus isolate on tomato was found to be systemic while the one on potato was non-systemic. The virus was present in all the tissues except xylem vessels in tomato, while it was often found only in the cortical cells of potato stems. Virions were detected in all cells of phloem tissue in tomato, while none were present in the case of potato. Plasmodesmata were present between phloem parenchyma and the phloem sieve tubes in infected tomato but none were present in infected potato. There were pronounced plasmodesmal changes in response to infection in tomato. These included dissolution of the desmotubule (central rod-like structure), making the plasmodesmata pore-like structures with a diameter of ca. 45 nm, which contained electron-dense material, presumably ribonucleoprotein of the virus. No such changes were found in infected potato. These results of comparative studies suggested that the virus was non-systemic in potato due to its non-loading into the phloem and consequently lacked long...

[1]  K. Oparka,et al.  Effect of virus infection on symplastic transport of fluorescent tracers in Nicotiana clevelandii leaf epidermis , 1990, Planta.

[2]  V. Citovsky,et al.  TRANSPORT OF PROTEINS AND NUCLEIC ACIDS THROUGH PLASMODESMATA. , 1997, Annual review of plant physiology and plant molecular biology.

[3]  D. Baulcombe,et al.  Cell-to-cell movement of potato virus X is associated with a change in the size-exclusion limit of plasmodesmata in trichome cells of Nicotiana clevelandii. , 1996, Virology.

[4]  M. Storms,et al.  The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells. , 1995, Virology.

[5]  W. J. Lucas,et al.  Symptom severity of beet western yellows virus strain ST9 is conferred by the ST9-associated RNA and is not associated with virus release from the phloem. , 1994, Virology.

[6]  M. Storms,et al.  Expression and subcellular location of the NSM protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. , 1994, Virology.

[7]  W. J. Lucas,et al.  Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[8]  A. Maule,et al.  Cauliflower mosaic virus gene I product (P1) forms tubular structures which extend from the surface of infected protoplasts. , 1993, Virology.

[9]  V. Citovsky,et al.  How do plant virus nucleic acids move through intercellular connections? , 1991, BioEssays : news and reviews in molecular, cellular and developmental biology.

[10]  B. Goodrick.  Restricted systemic movement of cowpea chlorotic mottle virus in soybean with nonnecrotic resistance , 1991 .

[11]  D. Reddy,et al.  Bud Necrosis: A Disease of Groundnut Caused by Tomato Spotted Wilt Virus , 1991 .

[12]  M. N. Singh,et al.  Potential reservoirs of tomato spotted wilt virus in the North West Himalayas. , 1990 .

[13]  J. Wellink,et al.  Evidence for the involvement of the 58K and 48K proteins in the intercellular movement of cowpea mosaic virus. , 1990 .

[14]  R. Hull.  The Movement of Viruses in Plants , 1989 .

[15]  M. Iwaki.  Silver Mottle Disease of watermelon caused by tomato spotted wilt virus. , 1984 .