Protein ligation: an enabling technology for the biophysical analysis of proteins

[1]  A. Otaka,et al.  Cysteine-derived s-protected oxazolidinones: potential chemical devices for the preparation of peptide thioesters. , 2006, Organic letters.

[2]  D. Schwarzer,et al.  Protein semisynthesis and expressed protein ligation: chasing a protein's tail. , 2005, Current opinion in chemical biology.

[3]  Mikkel A. Algire,et al.  Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation. , 2005, Molecular cell.

[4]  G. Marshall,et al.  Motion of Carboxyl Terminus of Gα Is Restricted upon G Protein Activation , 2005, Journal of Biological Chemistry.

[5]  Suren A Tatulian,et al.  Positioning membrane proteins by novel protein engineering and biophysical approaches. , 2005, Journal of molecular biology.

[6]  Liang Feng,et al.  Simple bioseparations using self-cleaving elastin-like polypeptide tags , 2005, Nature Methods.

[7]  A. Chilkoti,et al.  Self-cleavable stimulus responsive tags for protein purification without chromatography. , 2005, Journal of the American Chemical Society.

[8]  S. Züger,et al.  Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies , 2005, Nature Biotechnology.

[9]  T. Muir,et al.  Development of a tandem protein trans-splicing system based on native and engineered split inteins. , 2005, Journal of the American Chemical Society.

[10]  Phil Attard,et al.  Stabilization of native protein fold by intein-mediated covalent cyclization. , 2005, Journal of molecular biology.

[11]  J. Lorsch,et al.  A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon. , 2005, Molecular cell.

[12]  Michael E. Hahn,et al.  Manipulating proteins with chemistry: a cross-section of chemical biology. , 2005, Trends in biochemical sciences.

[13]  A. Gordon,et al.  Using protein-DNA chimeras to detect and count small numbers of molecules , 2005, Nature Methods.

[14]  R. MacKinnon,et al.  Glycine as a D-amino acid surrogate in the K(+)-selectivity filter. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[15]  Masasuke Yoshida,et al.  Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy. , 2004, Journal of the American Chemical Society.

[16]  P. Botti,et al.  Native chemical ligation through in situ O to S acyl shift. , 2004, Organic letters.

[17]  B. Chait,et al.  Domain-specific incorporation of noninvasive optical probes into recombinant proteins. , 2004, Journal of the American Chemical Society.

[18]  Mark P. de Caestecker,et al.  Structural Basis of Heteromeric Smad Protein Assembly in TGF-β Signaling , 2004 .

[19]  Xiang‐Qin Liu,et al.  Synthetic Two-piece and Three-piece Split Inteins for Protein trans-Splicing* , 2004, Journal of Biological Chemistry.

[20]  T. C. Evans,et al.  Mutational analysis of protein splicing, cleavage, and self-association reactions mediated by the naturally split Ssp DnaE intein. , 2004, Biochemistry.

[21]  J. D. Yoreo,et al.  Fmoc-Based Synthesis of Peptide α-Thioesters Using an Aryl Hydrazine Support† , 2004 .

[22]  S. Kent,et al.  A one-pot total synthesis of crambin. , 2004, Angewandte Chemie.

[23]  D. D. De Souza,et al.  Direct production of proteins with N-terminal cysteine for site-specific conjugation. , 2004, Bioconjugate chemistry.

[24]  Alexander Shekhtman,et al.  Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[25]  G. Kochendoerfer,et al.  Native Chemical Ligation of Hydrophobic Peptides in Lipid Bilayer Systems , 2004 .

[26]  G. Kochendoerfer,et al.  Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[27]  H. Kolb,et al.  The growing impact of click chemistry on drug discovery. , 2003, Drug discovery today.

[28]  T. Muir.  Semisynthesis of proteins by expressed protein ligation. , 2003, Annual review of biochemistry.

[29]  M. Uttamchandani,et al.  Cell-permeable small molecule probes for site-specific labeling of proteins. , 2003, Chemical communications.

[30]  Herbert Waldmann,et al.  Structure of Rab GDP-Dissociation Inhibitor in Complex with Prenylated YPT1 GTPase , 2003, Science.

[31]  Xiang‐Qin Liu,et al.  Split dnaE Genes Encoding Multiple Novel Inteins in Trichodesmium erythraeum* , 2003, Journal of Biological Chemistry.

[32]  T. Muir,et al.  Protein semi-synthesis in living cells. , 2003, Journal of the American Chemical Society.

[33]  Gun-Do Kim,et al.  Protein trans-splicing in transgenic plant chloroplast: Reconstruction of herbicide resistance from split genes , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[34]  Christof M Niemeyer,et al.  Synthesis of protein-nucleic acid conjugates by expressed protein ligation. , 2003, Chemical communications.

[35]  Jianjun Yang,et al.  Intein-mediated assembly of a functional β-glucuronidase in transgenic plants , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[36]  M. Belfort,et al.  Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing. , 2002, Nucleic acids research.

[37]  D. Payan,et al.  Retrovirally Delivered Random Cyclic Peptide Libraries Yield Inhibitors of Interleukin-4 Signaling in Human B Cells* , 2002, The Journal of Biological Chemistry.

[38]  R. MacKinnon,et al.  Semisynthesis and folding of the potassium channel KcsA. , 2002, Journal of the American Chemical Society.

[39]  B. Qin,et al.  Smad3 allostery links TGF-beta receptor kinase activation to transcriptional control. , 2002, Genes & development.

[40]  T. Muir,et al.  Protein splicing triggered by a small molecule. , 2002, Journal of the American Chemical Society.

[41]  T. Muir,et al.  Autoregulation of a bacterial σ factor explored by using segmental isotopic labeling and NMR , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[42]  Chi-Huey Wong,et al.  New Methods for Proteomic Research: Preparation of Proteins with N-Terminal Cysteines for Labeling and Conjugation This research was supported by the NIH (R37 GM44154). , 2002 .

[43]  Chi‐Huey Wong,et al.  New methods for proteomic research: preparation of proteins with N-terminal cysteines for labeling and conjugation. , 2002, Angewandte Chemie.

[44]  P. Dawson,et al.  Extending synthetic access to proteins with a removable acyl transfer auxiliary. , 2002, Journal of the American Chemical Society.

[45]  T. Muir,et al.  Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR. , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[46]  Francine B. Perler,et al.  InBase: the Intein Database , 2002, Nucleic Acids Res..

[47]  T. Muir,et al.  Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling. , 2001, Molecular cell.

[48]  D Cowburn,et al.  Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain. , 2001, Bioorganic & medicinal chemistry.

[49]  C. Bertozzi,et al.  Chemoselective approaches to glycoprotein assembly. , 2001, Accounts of chemical research.

[50]  K. Rose,et al.  Covalent capture: a new tool for the purification of synthetic and recombinant polypeptides. , 2001, Chemistry & biology.

[51]  T. Muir,et al.  Rescuing a destabilized protein fold through backbone cyclization. , 2001, Journal of molecular biology.

[52]  A. Plückthun,et al.  Cyclic Green Fluorescent Protein Produced in Vivo Using an Artificially Split PI-PfuI Intein from Pyrococcus furiosus * , 2001, The Journal of Biological Chemistry.

[53]  Y. Umezawa,et al.  Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing. , 2001, Analytical chemistry.

[54]  P. Dawson,et al.  Synthesis of peptides and proteins without cysteine residues by native chemical ligation combined with desulfurization. , 2001, Journal of the American Chemical Society.

[55]  S. Benkovic,et al.  Structural requirements for the biosynthesis of backbone cyclic peptide libraries. , 2001, Chemistry & biology.

[56]  T. Muir,et al.  Synthesis of Multi-Domain Proteins Using Expressed Protein Ligation: Strategies for Segmental Isotopic Labeling of Internal Regions , 2000 .

[57]  D. Hilvert,et al.  Facile, fmoc-compatible solid-phase synthesis of peptide C-terminal thioesters. , 2000, Organic letters.

[58]  C. Bertozzi,et al.  A "traceless" Staudinger ligation for the chemoselective synthesis of amide bonds. , 2000, Organic letters.

[59]  R. Raines,et al.  Staudinger ligation: a peptide from a thioester and azide. , 2000, Organic letters.

[60]  T. C. Evans,et al.  Protein trans-Splicing and Cyclization by a Naturally Split Intein from the dnaE Gene ofSynechocystis Species PCC6803* , 2000, The Journal of Biological Chemistry.

[61]  S. Chong,et al.  Fusions to self-splicing inteins for protein purification. , 2000, Methods in enzymology.

[62]  H. Paulus,et al.  Protein splicing and related forms of protein autoprocessing. , 2000, Annual review of biochemistry.

[63]  Carolyn R. Bertozzi,et al.  Fmoc-Based Synthesis of Peptide-αThioesters: Application to the Total Chemical Synthesis of a Glycoprotein by Native Chemical Ligation , 1999 .

[64]  F. Albericio,et al.  Backbone Amide Linker (BAL) Strategy for N(alpha)()-9-Fluorenylmethoxycarbonyl (Fmoc) Solid-Phase Synthesis of Unprotected Peptide p-Nitroanilides and Thioesters(1). , 1999, The Journal of organic chemistry.

[65]  A. Pessi,et al.  Solid Phase Synthesis of Peptide C-Terminal Thioesters by Fmoc/t-Bu Chemistry , 1999 .

[66]  S. Benkovic,et al.  Production of cyclic peptides and proteins in vivo. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[67]  Y. Kyōgoku,et al.  NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation. , 1999, Biochemistry.

[68]  Andreas Plückthun,et al.  Circular β‐lactamase: stability enhancement by cyclizing the backbone , 1999 .

[69]  David W. Wood,et al.  A genetic system yields self-cleaving inteins for bioseparations , 1999, Nature Biotechnology.

[70]  J. Griffin,et al.  Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[71]  G. Kochendoerfer,et al.  Total chemical synthesis of the integral membrane protein influenza A virus M2: role of its C-terminal domain in tetramer assembly. , 1999, Biochemistry.

[72]  A. Pessi,et al.  Engineering and Chemical Synthesis of a Transmembrane Protein: The HCV Protease Cofactor Protein NS4A , 1999 .

[73]  T. C. Evans,et al.  The Cyclization and Polymerization of Bacterially Expressed Proteins Using Modified Self-splicing Inteins* , 1999, The Journal of Biological Chemistry.

[74]  G. Beligere,et al.  Conformationally Assisted Protein Ligation Using C-Terminal Thioester Peptides , 1999 .

[75]  T. Muir,et al.  Biosynthesis of a Head-to-Tail Cyclized Protein with Improved Biological Activity , 1999 .

[76]  T. C. Evans,et al.  Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. , 1999, Gene.

[77]  T. C. Evans,et al.  The in Vitro Ligation of Bacterially Expressed Proteins Using an Intein from Methanobacterium thermoautotrophicum * , 1999, The Journal of Biological Chemistry.

[78]  T. Muir,et al.  Insertion of a Synthetic Peptide into a Recombinant Protein Framework: A Protein Biosensor , 1999 .

[79]  D Cowburn,et al.  Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[80]  Y. Le Dréan,et al.  Purification of Proteins Fused to Either the Amino or Carboxy Terminus of the Mycobacterium xenopi Gyrase A Intein , 1999 .

[81]  A. Plückthun,et al.  Circular beta-lactamase: stability enhancement by cyclizing the backbone. , 1999, FEBS letters.

[82]  T. Muir,et al.  Chemical ligation of unprotected peptides directly from a solid support. , 2009, The journal of peptide research : official journal of the American Peptide Society.

[83]  Jack Benner,et al.  Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. , 1998, Nucleic acids research.

[84]  T. C. Evans,et al.  Semisynthesis of cytotoxic proteins using a modified protein splicing element , 1998, Protein science : a publication of the Protein Society.

[85]  Z. Hu,et al.  Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[86]  T. Muir,et al.  Expressed protein ligation: a general method for protein engineering. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[87]  Toshio Yamazaki,et al.  Segmental Isotope Labeling for Protein NMR Using Peptide Splicing , 1998 .

[88]  Ming-Qun Xu,et al.  Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein* , 1998, The Journal of Biological Chemistry.

[89]  B. Chait,et al.  The structure of the potassium channel: molecular basis of K+ conduction and selectivity. , 1998, Science.

[90]  K V Mills,et al.  Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[91]  M. Southworth,et al.  Control of protein splicing by intein fragment reassembly , 1998, The EMBO journal.

[92]  H. Paulus,et al.  Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments. , 1998, Gene.

[93]  M. Belfort,et al.  Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[94]  F. Perler,et al.  Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. , 1997, Gene.

[95]  Philip E. Dawson,et al.  Modulation of Reactivity in Native Chemical Ligation through the Use of Thiol Additives , 1997 .

[96]  G. Verdine,et al.  The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complex. , 1996, Chemistry & biology.

[97]  F. Perler,et al.  The mechanism of protein splicing and its modulation by mutation. , 1996, The EMBO journal.

[98]  S. Kent,et al.  EXTENDING THE APPLICABILITY OF NATIVE CHEMICAL LIGATION , 1996 .

[99]  T. Muir,et al.  Synthesis of proteins by native chemical ligation. , 1994, Science.

[100]  K. Rose,et al.  Construction of protein analogues by site-specific condensation of unprotected fragments. , 1992, Bioconjugate chemistry.

[101]  S. Kent,et al.  Constructing proteins by dovetailing unprotected synthetic peptides: backbone-engineered HIV protease. , 1992, Science.

[102]  P Argos,et al.  Oligopeptide biases in protein sequences and their use in predicting protein coding regions in nucleotide sequences , 1988, Proteins.

[103]  R. B. Merrifield,et al.  Chemical synthesis of peptides and proteins. , 1970, Annual review of biochemistry.