Natural variability of biochemical biomarkers in the macro‐zoobenthos: Dependence on life stage and environmental factors

Biomarkers are widely used in ecotoxicology as indicators of exposure to toxicants. However, their ability to provide ecologically relevant information remains controversial. One of the major problems is understanding whether the measured responses are determined by stress factors or lie within the natural variability range. In a previous work, the natural variability of enzymatic levels in invertebrates sampled in pristine rivers was proven to be relevant across both space and time. In the present study, the experimental design was improved by considering different life stages of the selected taxa and by measuring more environmental parameters. The experimental design considered sampling sites in 2 different rivers, 8 sampling dates covering the whole seasonal cycle, 4 species from 3 different taxonomic groups (Plecoptera, Perla grandis; Ephemeroptera, Baetis alpinus and Epeorus alpicula; Tricoptera, Hydropsyche pellucidula), different life stages for each species, and 4 enzymes (acetylcholinesterase, glutathione S‐transferase, alkaline phosphatase, and catalase). Biomarker levels were related to environmental (physicochemical) parameters to verify any kind of dependence. Data were statistically elaborated using hierarchical multilevel Bayesian models. Natural variability was found to be relevant across both space and time. The results of the present study proved that care should be paid when interpreting biomarker results. Further research is needed to better understand the dependence of the natural variability on environmental parameters. Environ Toxicol Chem 2017;36:3158–3167. © 2017 SETAC

[1]  B. Quinn,et al.  Seasonal variations of biomarker responses in the marine blue mussel (Mytilus spp.). , 2013, Marine pollution bulletin.

[2]  M. Bebianno,et al.  Spatial and seasonal biomarker responses in the clam Ruditapes decussatus , 2013, Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals.

[3]  P. Calow,et al.  Promises and problems for the new paradigm for risk assessment and an alternative approach involving predictive systems models , 2012, Environmental toxicology and chemistry.

[4]  M. Bebianno,et al.  Application of an integrated biomarker response index (IBR) to assess temporal variation of environmental quality in two Portuguese aquatic systems , 2012 .

[5]  M. Solé,et al.  Natural variability of hepatic biomarkers in Mediterranean deep-sea organisms. , 2012, Marine environmental research.

[6]  M. L. Martín-Díaz,et al.  Chronic contamination assessment integrating biomarkers' responses in transplanted mussels—A seasonal monitoring , 2012, Environmental toxicology.

[7]  C. Martyniuk,et al.  Omics in aquatic toxicology: Not just another microarray , 2011, Environmental toxicology and chemistry.

[8]  P. J. Van den Brink,et al.  Potential application of ecological models in the European environmental risk assessment of chemicals I: Review of protection goals in EU directives and regulations , 2010, Integrated environmental assessment and management.

[9]  William H. Benson,et al.  Integrating Omic Technologies into Aquatic Ecological Risk Assessment and Environmental Monitoring: Hurdles, Achievements, and Future Outlook , 2009, Environmental health perspectives.

[10]  D. Barceló,et al.  Combined use of biomarkers and in situ bioassays in Daphnia magna to monitor environmental hazards of pesticides in the field , 2007, Environmental toxicology and chemistry.

[11]  M. Peck,et al.  Biomarker responses of the estuarine brown shrimp Crangon crangon L. to non-toxic stressors: Temperature, salinity and handling stress effects , 2006 .

[12]  Gerald T Ankley,et al.  Toxicogenomics in regulatory ecotoxicology. , 2006, Environmental science & technology.

[13]  B. Rossaro,et al.  Biomarkers in Caddisfly Larvae of the Species Hydropsyche pellucidula (Curtis, 1834) (Trichoptera: Hydropsychidae) Measured in Natural Populations and after Short Term Exposure to Fenitrothion , 2006, Bulletin of environmental contamination and toxicology.

[14]  Valery E Forbes,et al.  The use and misuse of biomarkers in ecotoxicology , 2006, Environmental toxicology and chemistry.

[15]  C. L. Rowe,et al.  Tools for Assessing Contaminant Exposure and Effects in Reptiles , 2005 .

[16]  Simone Pfeifer,et al.  Effect of temperature and salinity on acetylcholinesterase activity, a common pollution biomarker, in Mytilus sp. from the south-western Baltic Sea , 2005 .

[17]  P. Zwick.  Key to the West Palaearctic genera of stoneflies (Plecoptera) in the larval stage , 2004 .

[18]  B. Rossaro,et al.  Evaluation of enzyme biomarkers in freshwater invertebrates from Taro and Ticino river, Italy , 2004 .

[19]  A. Callaghan,et al.  Intraclonal variability in Daphnia acetylcholinesterase activity: The implications for its applicability as a biomarker , 2003, Environmental toxicology and chemistry.

[20]  M. Crane,et al.  Effect of temperature and pirimiphos methyl on biochemical biomarkers in Chironomus riparius Meigen. , 2002, Ecotoxicology and environmental safety.

[21]  M. Crane,et al.  Variability in acetylcholinesterase and glutathione S‐transferase activities in Chironomus riparius meigen deployed in situ at uncontaminated field sites , 2001, Environmental toxicology and chemistry.

[22]  J. Payne.  Mixed function oxidases in marine organisms in relation to petroleum hydrocarbon metabolism and detection , 1977 .

[23]  J. Widdows,et al.  A cytochemical and a biochemical index of stress in Mytilus edulis L. , 1976 .

[24]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[25]  D. Peakall.  Effects of toxaphene on hepatic enzyme induction and circulating steroid levels in the rat. , 1976, Environmental health perspectives.

[26]  W B Jakoby,et al.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. , 1974, The Journal of biological chemistry.

[27]  M. Vighi,et al.  Natural variability of enzymatic biomarkers in freshwater invertebrates , 2016, Environmental Science and Pollution Research.

[28]  P. Kestemont,et al.  Combined effects of deltamethrin, temperature and salinity on oxidative stress biomarkers and acetylcholinesterase activity in the black tiger shrimp (Penaeus monodon). , 2012, Chemosphere.