Varietal Differences in Embryogenic Ability in Anther Culture of Chinese Cabbage (Brassica campestris ssp. pekinensis)

The varietal differences in embryogenic ability in anther culture of Chinese cabbage, Brassica campestris ssp. pekinensis, were investigated. In the first experiment, the anthers of B. campestris were incubated in the modified B5 medium (KELLER and ARMSTRONG 1979) at 30°C for 14 days and then maintained at 25°C in the dark. Only a few embryos were ob-served in a limited number of cultivars in this treatment. In contrast, embryos were formed efficiently by the treatment at 35'C for one day. The embryogenic ability from anthers was remarkably different among the cultivars. Among the cultivars of B. campestris, the anthers of 'ASO Ichigo' belonging to the Kenshin group produced embryos most efficiently. The second experiment was conducted to know the embryogenic ability of cultivars in the Kenshin group. Although some varietal differences in the embryogenic ability were observed, one of the cultivars exhibited the highest culture efficiency (expected yield of embryos from 1, 000 anthers cultured) of over 10, 000. Comparing the data of two experiments, the Kenshin group were thought to have a high embryogenic ability in anther culture of Chinese cabbage.

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