Supercritical fluid chromatography/chemical ionization/mass spectrometry of some anticancer drugs in a thermospray ion source.

A packed-column supercritical-fluid chromatograph was interfaced with a mass spectrometer via a modification of a thermospray probe. This modification allowed a capillary restrictor for the supercritical fluid (CO2) and reagent gas for chemical ionization to be introduced directly into a thermospray source. Chemical ionization conditions were observed when either the filament or discharge electrode was used and the source pressure was above 0.5 torr. The discharge electrode produced more efficient ionization, resulting in approximately a tenfold larger signal than that observed in the filament mode. The usefulness of this instrumentation was demonstrated on several anticancer drugs. Methanol positive ion chemical ionization (PICI) spectra were recorded for cyclophosphamide, diaziquone, mitomycin C and thiotepa. Methane PICI spectra of thiotepa were obtained in the absence of methanol as a mobile-phase modifier. A 50 ng on-column injection of diaziquone produced approximately a 6:1 signal to noise ratio in the scanning mode.

[1]  S. Musser,et al.  Liquid chromatography-thermospray mass spectrometry of DNA adducts formed with mitomycin C, porfiromycin and thiotepa. , 1989, Journal of chromatography.

[2]  M. Ratain,et al.  Plasma pharmacokinetics of high-dose oral melphalan in patients treated with trialkylator chemotherapy and autologous bone marrow reinfusion. , 1989, Cancer research.

[3]  E. M. Calvey,et al.  Capillary supercritical fluid chromatography/negative ion chemical ionization mass spectrometry of trichothecenes. , 1989, Biomedical & environmental mass spectrometry.

[4]  J. R. Perkins,et al.  Packed column supercritical fluid chromatography mass spectrometry using a thermospray source in the filament-on mode , 1988 .

[5]  J. Chapman,et al.  Coupled supercritical fluid chromatography/mass spectrometry using a thermospray source , 1988 .

[6]  S. Purl,et al.  A phase I-II study of bialkylator chemotherapy, high-dose thiotepa, and cyclophosphamide with autologous bone marrow reinfusion in patients with advanced cancer. , 1987, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[7]  Richard D. Smith,et al.  Mass spectrometer interface for microbore and high flow rate capillary supercritical fluid chromatography with splitless injection , 1987 .

[8]  P. Callery,et al.  The chemical reduction of diaziquone: products and free radical intermediates. , 1987, Chemico-biological interactions.

[9]  H. Schwartz,et al.  Integral Pressure Restrictor for Capillary SFC , 1986 .

[10]  D. Later,et al.  Analysis of various classes of drugs by capillary supercritical fluid chromatography. , 1986, Journal of chromatographic science.

[11]  C. M. White,et al.  Supercritical fluid chromatography and some of its applications: a review , 1986 .

[12]  J. Henion,et al.  Supercritical fluid chromatography of polar drugs using small-particle packed columns with mass spectrometric detection. , 1985, Analytical chemistry.

[13]  M. Vestal,et al.  Thermospray liquid chromatograph/mass spectrometer interface with direct electrical heating of the capillary. , 1985, Analytical chemistry.

[14]  B. W. Wright,et al.  Rapid and high resolution capillary supercritical fluid chromatography (SFC) and SFC/MS of trichothecene mycotoxins. , 1985, Journal of chromatographic science.

[15]  B. W. Wright,et al.  Rapid and efficient capillary column supercritical fluid chromatography with mass spectrometric detection , 1984 .

[16]  Richard D. Smith,et al.  Direct fluid injection interface for capillary supercritical fluid chromatography-mass spectrometry , 1982 .

[17]  Milton L. Lee,et al.  Capillary column supercritical fluid chromatography mass spectrometry , 1982 .