Stokes shift emission spectroscopy of key biomolecules in human tissues

The Stokes-shifted emission spectra were measured for various photoactive biomolecules such as tryptophan, collagen, NADH and flavin in aqueous solution and tissue. Information is obtained on the molecular activity in the tissue. This new approach allows for the extraction of information not obtained from excitation and/or fluorescence spectroscopy for a single spectral scan.