Application and Development of Bt Insect Resistance Genes in Rice Breeding

As pests are an important factor in reducing crop yields, pest control is an important measure in preventing reductions in crop yields. With the aim of ending the use of chemical pesticides, biological control and genetically modified methods are now considered more reasonable pest control strategies. The bacterium Bacillus thuringiensis (Bt) can produce crystal proteins that have specific toxicity to lepidopteran insects, and so it has been applied as a microbial insecticide in the control of crop pests for several decades. With the development of plant genetic engineering, Bt genes encoding insecticidal crystal protein have been introduced into many crop species for pest control. This article indicates that, after years of experiments and research, Bt transgenic rice is close to becoming a commercial insect-resistant rice, and many studies have shown that transgenic rice has pronounced abilities in the control of pests such as yellow stem borers (Scirpophaga incertulas, YSB), striped stem borers (Chilo suppressalis, SSB), and rice leaf rollers (Cnaphalocrocis medinalis, RLR); moreover, it does not obviously differ from non-transgenic rice in terms of safety. This paper suggests that transgenic Bt rice has application potential and commercial value.

[1]  J. Oliveira,et al.  Advances in Environmentally Friendly Techniques and Circular Economy Approaches for Insect Infestation Management in Stored Rice Grains , 2023, Foods.

[2]  H. Rouached,et al.  Genetically engineered crops for sustainably enhanced food production systems , 2022, Frontiers in Plant Science.

[3]  C. Gardner,et al.  Environmental fate and behaviour of antibiotic resistance genes and small interference RNAs released from genetically modified crops , 2022, Journal of applied microbiology.

[4]  Jianling Deng,et al.  [Accumulation of Cry proteins in soil released from Bt rice after planting for multiple years]. , 2022, Ying yong sheng tai xue bao = The journal of applied ecology.

[5]  Suresh Panneerselvam,et al.  A structure-based nomenclature for Bacillus thuringiensis and other bacteria-derived pesticidal proteins. , 2020, Journal of invertebrate pathology.

[6]  Zhicheng Shen,et al.  Characterization of transgenic rice expressing fusion protein Cry1Ab/Vip3A for insect resistance , 2018, Scientific Reports.

[7]  Srinath Tamirisa,et al.  Expression of hybrid fusion protein (Cry1Ac::ASAL) in transgenic rice plants imparts resistance against multiple insect pests , 2018, Scientific Reports.

[8]  G. Yè,et al.  Effects of Transgenic Bt Rice on Nontarget Rhopalosiphum maidis (Homoptera: Aphididae) , 2016, Environmental Entomology.

[9]  J. Zhao,et al.  Prey-mediated effects of transgenic cry2Aa rice on the spider Hylyphantes graminicola, a generalist predator of Nilapavarta lugens , 2015, BioControl.

[10]  Manqun Wang,et al.  Transgenic cry1C(⁎) gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae). , 2015, Ecotoxicology and environmental safety.

[11]  Yu-fa Peng,et al.  A 90 day safety assessment of genetically modified rice expressing Cry1Ab/1Ac protein using an aquatic animal model. , 2015, Journal of agricultural and food chemistry.

[12]  H. Hua,et al.  No impact of transgenic cry2Aa rice on Anagrus nilaparvatae, an egg parasitoid of Nilaparvata lugens, in laboratory tests , 2015 .

[13]  J. Romeis,et al.  Consumption of Bt rice pollen containing Cry1C or Cry2A does not pose a risk to Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) , 2015, Scientific Reports.

[14]  G. Yè,et al.  Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions , 2014 .

[15]  Yu-fa Peng,et al.  Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields , 2014, Ecotoxicology.

[16]  X. Jia,et al.  A two-generation reproduction study with transgenic Bt rice TT51 in Wistar rats. , 2014, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[17]  G. Yè,et al.  Impacts of Bt Rice Expressing Cry1C or Cry2A Protein on the Performance of Nontarget Leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), Under Laboratory and Field Conditions , 2014, Environmental entomology.

[18]  H. Xu,et al.  Effects of 90-day feeding of transgenic Bt rice TT51 on the reproductive system in male rats. , 2013, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[19]  J. Romeis,et al.  Bt rice expressing Cry2Aa does not cause direct detrimental effects on larvae of Chrysoperla sinica , 2013, Ecotoxicology.

[20]  Yun Luo,et al.  Effects of genetically modified T2A-1 rice on the GI health of rats after 90-day supplement , 2013, Scientific Reports.

[21]  J. Romeis,et al.  Consumption of Bt rice pollen expressing Cry2Aa does not cause adverse effects on adult Chrysoperla sinica Tjeder (Neuroptera: Chrysopidae) , 2012 .

[22]  Wentao Xu,et al.  Safety assessment of transgenic Bacillus thuringiensis rice T1c‐19 in Sprague–Dawley rats from metabonomics and bacterial profile perspectives , 2012, IUBMB life.

[23]  Wentao Xu,et al.  Effects of genetically modified T2A-1 rice on faecal microflora of rats during 90 day supplementation. , 2011, Journal of the science of food and agriculture.

[24]  Wei Tang,et al.  Development and characterisation of transgenic rice expressing two Bacillus thuringiensis genes. , 2011, Pest management science.

[25]  Wentao Xu,et al.  Metabonomics study of transgenic Bacillus thuringiensis rice (T2A-1) meal in a 90-day dietary toxicity study in rats. , 2011, Molecular bioSystems.

[26]  J. Sangha,et al.  Production of marker-free transgenic rice expressing tissue-specific Bt gene , 2010, Plant Cell Reports.

[27]  S. Caccia,et al.  Binding Site Alteration Is Responsible for Field-Isolated Resistance to Bacillus thuringiensis Cry2A Insecticidal Proteins in Two Helicoverpa Species , 2010, PloS one.

[28]  Qifa Zhang,et al.  Review and prospect of transgenic rice research , 2009 .

[29]  Xiao-ming Zhang,et al.  Development of Marker-Free Transgenic Cry1Ab Rice with Lepidopteran Pest Resistance by Agrobacterium Mixture-Mediated Co-transformation , 2009 .

[30]  Li Liu,et al.  Development of insect-resistant transgenic rice with Cry1C*-free endosperm. , 2009, Pest management science.

[31]  D. Crowder,et al.  Insect resistance to Bt crops: evidence versus theory , 2008, Nature Biotechnology.

[32]  Kongming Wu,et al.  Degradation of Cry1Ac Protein Within Transgenic Bacillus thuringiensis Rice Tissues Under Field and Laboratory Conditions , 2007, Environmental entomology.

[33]  Kongming Wu,et al.  Efficacy of transgenic rice expressing Cry1Ac and CpTI against the rice leaffolder, Cnaphalocrocis medinalis (Guenée). , 2007, Journal of invertebrate pathology.

[34]  H. Li,et al.  Comparison of nutritional quality between Chinese indica rice with sck and cry1Ac genes and its nontransgenic counterpart. , 2007, Journal of food science.

[35]  A. Shelton,et al.  Impacts of transgenic cry1Ab rice on non-target planthoppers and their main predator Cyrtorhinus lividipennis (Hemiptera: Miridae)—A case study of the compatibility of Bt rice with biological control , 2007 .

[36]  Kongming Wu,et al.  Cross-Resistance Studies of Cry1Ac-Resistant Strains ofHelicoverpa armigera (Lepidoptera: Noctuidae) to Cry2Ab , 2007, Journal of economic entomology.

[37]  Kaveh Emami,et al.  A 90-day safety study in Wistar rats fed genetically modified rice expressing snowdrop lectin Galanthus nivalis (GNA). , 2007, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[38]  Weiqi Wang,et al.  Cry1Ab protein from Bt transgenic rice does not residue in rhizosphere soil. , 2006, Environmental pollution.

[39]  Jia Hua Cheng,et al.  Effects of Cry1Ab Toxin on Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) Through Its Prey, Nilaparvata lugens Stål (Homoptera: Delphacidae), Feeding on Transgenic Bt Rice , 2006 .

[40]  Wei Tang,et al.  Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene , 2006, Molecular Breeding.

[41]  S. Datta,et al.  Field Assessment of the Effects of Transgenic Rice Expressing a Fused Gene of cry1Ab and cry1Ac from Bacillus thuringiensis Berliner on Nontarget Planthopper and Leafhopper Populations , 2006 .

[42]  Wei Tang,et al.  Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests , 2005, Theoretical and Applied Genetics.

[43]  J. González-Cabrera,et al.  Binding analyses of Cry1Ab and Cry1Ac with membrane vesicles from Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis. , 2004, Biochemical and biophysical research communications.

[44]  N. P. Sarma,et al.  Development of stem borer resistant transgenic parental lines involved in the production of hybrid rice. , 2004, Journal of biotechnology.

[45]  Wu Wei-xiang,et al.  Bt-transgenic rice straw affects the culturable microbiota and dehydrogenase and phosphatase activities in a flooded paddy soil , 2004 .

[46]  Q. Ye,et al.  COMPARATIVE STUDIES ON MAJOR NUTRITIONAL COMPONENTS AND PHYSICOCHEMICAL PROPERTIES OF THE TRANSGENIC RICE WITH A SYNTHETIC Cry1Ab GENE FROM BACILLUS THURINGIENSIS , 2003 .

[47]  S. Raina,et al.  Elite Indica Transgenic Rice Plants Expressing Modified Cry1Ac Endotoxin of Bacillus Thuringiensis Show Enhanced Resistance to Yellow Stem Borer (Scirpophaga Incertulas) , 2002, Transgenic Research.

[48]  Zhonghua Wang,et al.  Toxicological evaluation of transgenic rice flour with a synthetic cry1Ab gene from Bacillus thuringiensis , 2002 .

[49]  S. Riazuddin,et al.  Expression of synthetic Cry1Ab and Cry1Ac genes in basmati rice (Oryza sativa L.) variety 370 via Agrobacterium-mediated transformation for the control of the european corn borer (Ostrinia nubilalis) , 2002, In Vitro Cellular & Developmental Biology - Plant.

[50]  Y. Li,et al.  Inheritance and expression of the cry1Ab gene in Bt (Bacillus thuringiensis) transgenic rice , 2002, Theoretical and Applied Genetics.

[51]  A. Shelton,et al.  Different Cross-Resistance Patterns in the Diamondback Moth (Lepidoptera: Plutellidae) Resistant to Bacillus thuringiensis Toxin Cry1C , 2001, Journal of Economic Entomology.

[52]  I. Altosaar,et al.  Field Evaluation of Resistance of Transgenic Rice Containing a Synthetic cry1Ab Gene from Bacillus thuringiensis Berliner to Two Stem Borers , 2001, Journal of economic entomology.

[53]  Cai-guo Xu,et al.  Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin , 2000, Nature Biotechnology.

[54]  T. Malvar,et al.  Integrative Model for Binding of Bacillus thuringiensis Toxins in Susceptible and Resistant Larvae of the Diamondback Moth (Plutella xylostella) , 1999, Applied and Environmental Microbiology.

[55]  N. Crickmore,et al.  Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins , 1998, Microbiology and Molecular Biology Reviews.

[56]  I. Altosaar,et al.  Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[57]  S. Datta,et al.  Transgenic fertile japonica rice plants expressing a modified cryIA(b) gene resistant to yellow stem borer , 1997, Plant Cell Reports.

[58]  Gurdev S. Khush,et al.  Enhanced resistance to two stem borers in an aromatic rice containing a synthetic cryIA(b) gene , 1997, Molecular Breeding.

[59]  A. Basu,et al.  Transgenic elite indica rice plants expressing CryIAc delta-endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas). , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[60]  A. Klöti,et al.  Transgenic Indica Rice Breeding Line IR58 Expressing a Synthetic crylA(b) Gene from Bacillus thuringiensis Provides Effective Insect Pest Control , 1996, Bio/Technology.

[61]  H. R. Whiteley,et al.  Insecticidal crystal proteins of Bacillus thuringiensis , 1989 .

[62]  M. Chilton,et al.  Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny , 1983, Cell.

[63]  H. R. Whiteley,et al.  Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[64]  Xiaoguang Yang,et al.  Long-term toxicity study on transgenic rice with Cry1Ac and sck genes. , 2014, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[65]  J. B.J. van Rensburg,et al.  First report of field resistance by the stem borer, Busseola fusca (Fuller) to Bt-transgenic maize , 2007 .

[66]  K. Shimamoto,et al.  Insect Resistant Rice Generated by Introduction of a Modified δ-endotoxin Gene of Bacillus thuringiensis , 1993, Bio/Technology.