Computed tomography scanning for three-dimensional imaging and complexity analysis of developing root systems

To improve our understanding of the role of root systems in soil-based resource acquisition by plants and eventually model it completely, root system complexity must be quantified, in addition to other morphometric traits. In this note, we introduce a new approach in which computed tomography (CT) scan data are collected on crop root systems in three-dimensional (3-D) space nondestructively and noninvasively, thus allowing for repeated measurements and a relevant complexity analysis of root systems. The experimental crop is maize (Zea mays L.). Four potted seedlings were CT scanned under wet soil conditions on the day of emergence, and each of the two following days. Specifically, a high-resolution X-ray CT scanner formerly used for medical purposes produced 3 × 500 CT images of 0.1 mm thick cross-sections for each seedling. The fractal dimension of each root system on each day was estimated on a skeletonized 3-D image reconstructed from CT scan data. We found that the mean fractal dimension value was not...

[1]  P. Dutilleul,et al.  Effects of Plant Population Density and Intercropping with Soybean on the Fractal Dimension of Corn Plant Skeletal Images , 2000 .

[2]  Benoit B. Mandelbrot,et al.  Fractal Geometry of Nature , 1984 .

[3]  J. D. Corbit,et al.  Fractal dimension as a quantitative measure of complexity in plant development , 1995, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[4]  Arthur W. Toga,et al.  Efficient Skeletonization of Volumetric Objects , 1999, IEEE Trans. Vis. Comput. Graph..

[5]  Akira Yamauchi,et al.  Fractal Analysis of Plant Root Systems , 1989 .

[6]  F. I. Woodward,et al.  The root system architecture and development of Senecio vulgaris in elevated CO_2 and drought , 1992 .

[7]  K. Gross,et al.  Seedling growth and root morphology of plants with different life‐histories , 1992 .

[8]  David J. Hand,et al.  Analysis of Repeated Measures , 1990 .

[9]  G. Berntson,et al.  Root Systems and Fractals: How Reliable are Calculations of Fractal Dimensions? , 1994 .

[10]  Anne M. Parkhurst,et al.  Fractal Analysis for Morphological Description of Corn Roots under Nitrogen Stress , 1993 .

[11]  Alastair H. Fitter,et al.  Characteristics and Functions of Root Systems , 2002 .

[12]  L. Aylmore.  Application of Computer Assisted Tomography to Soil‐Plant‐Water Studies: An Overview , 1994 .

[13]  Chris Moran,et al.  X-ray computed tomography to quantify tree rooting spatial distributions , 1999 .

[14]  J. Lynch,et al.  Growth and architecture of seedling roots of common bean genotypes , 1993 .

[15]  P. P. Feret,et al.  Using Fractal Geometry to Quantify Loblolly Pine Seedling Root System Architecture , 1993 .

[16]  J. Hopmans,et al.  Three dimensional imaging of plant roots in situ with X-ray Computed Tomography , 1997, Plant and Soil.

[17]  K. L. Nielsen,et al.  Fractal geometry of bean root systems: correlations between spatial and fractal dimension. , 1997, American journal of botany.

[18]  G. Johnson,et al.  Use of Magnetic Resonance Imaging in the Study of Plants and Soils , 1994 .

[19]  P. Dutilleul,et al.  Morphology and fractal dimension of root systems of maize hybrids bearing the leafy trait , 2003 .

[20]  Dr. Wolfgang Böhm.  Methods of Studying Root Systems , 1979, Ecological Studies.

[21]  W. Stuppy,et al.  Three-dimensional analysis of plant structure using high-resolution X-ray computed tomography. , 2003, Trends in plant science.

[22]  P. Dutilleul,et al.  Branching out with a CT scanner. , 2005, Trends in plant science.

[23]  Pierre Dutilleul,et al.  Soybean canopy development as affected by population density and intercropping with corn : Fractal analysis in comparison with other quantitative approaches , 1999 .

[24]  R. Ketcham,et al.  Acquisition, optimization and interpretation of X-ray computed tomographic imagery: applications to the geosciences , 2001 .

[25]  Alastair H. Fitter,et al.  Architectural analysis of plant root systems , 1992 .

[26]  Pierre Dutilleul,et al.  Advances in the implementation of the box-counting method of fractal dimension estimation , 1999, Appl. Math. Comput..

[27]  Ali Shahrokni,et al.  Fast skeletonization algorithm for 3D elongated objects , 2001, SPIE Medical Imaging.