Determination of protein with Thioguanine (6-TG) as a probe by synchronous fluorescence technique

Abstract The synchronous fluorescence technique was developed for the determination of human serum albumin (HSA) in human body fluids with Thioguanine (6-TG) as a molecular probe. Under the optimal experimental conditions, the synchronous fluorescence peak of HSA–6-TG system is located at about 301 nm and the enhancement synchronous intensity was proportion to the concentration of HSA. The calibration graphs are linear over the range of 0.69–552.0 μg mL −1 with a correlation coefficient ( R ) of 0.9994. The detection limit was 0.133 μg mL −1 . It was successfully applied to determine the protein in human body fluids including serum, urine and saliva samples with 6-TG as probe with a satisfying result. In the analysis of human body liquids samples, the relative standard deviations ( RSDs ) were 0.55–3.31%, which obtained from 6 replicate determinations and recoveries were in the range of 97.0–104.1%.

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