STAINS COMPATIBLE WITH DIPPING RADIOAUTOGRAPHY.

Paraffin sections of 13 different kinds of mouse tissues, containing tritiated thymidine, were used to test stains applied either before or after application of the nuclear emulsion by dipping. Criteria used to determine compatibility were good histological definition with moderate gelatin coloration, sharp contrast to silver granules and no artifact or bleaching. Stains which worked best when applied prior to dipping included Feulgen-fast green, chronium hematoxylin-phloxine, and aldehyde fuchsin-PAS. Stains which worked best when performed after photographic development included celestin blue-Mayer's haemalum, metanil yellow-iron hematoxylin, lithium carmine-picric acid, Weigert acid-iron hematoxylin, alum cochineal, methyl green-pyronin, indigo carmine-picric acid, methylene blue-azure A, toluidine blue, Nissl and Cason. “Combination” stains which worked well when tissues were partially stained before dipping and completed after development included trichrome-PAS, luxol fast blue-PAS, hematoxylin-eosin...

[1]  D. L. Joftes.  Radioautography, principles and procedures. , 1963, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[2]  U. Stenram.  Loss of silver grains from radioautographs stained by gallocyanin-chrome alum. , 1962, Stain technology.

[3]  M. Mortreuil-Langlois.  Staining sections coated with radiographic emulsion: a nuclear fast red, indigo-carmine sequence. , 1962, Stain technology.

[4]  L. Bélanger.  Staining processed radioautographs. , 1961, Stain technology.

[5]  H. Quastler,et al.  COMBINED AUTORADIOGRAPHY AND ELECTRON MICROSCOPY OF THIN SECTIONS OF INTESTINAL EPITHELIAL CELLS OF THE MOUSE LABELED WITH H3-THYMIDINE , 1961, The Journal of biophysical and biochemical cytology.

[6]  P. Pellerin.  [The technic of anatomical radioutography at the temperature of liquid nitrogen]. , 1961, Pathologie et biologie.

[7]  H. Elftman.  Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. , 1959, Stain technology.

[8]  E. Kaminski.  Histological processing in autoradiography: loss of radioactivity. , 1955, Stain technology.

[9]  V. Witten,et al.  New histologic technics for autoradiography. , 1953, Laboratory investigation; a journal of technical methods and pathology.

[10]  A. G. Everson.  Histochemistry: Theoretical and Applied , 1953 .

[11]  J. E. Cason,et al.  A rapid one-step Mallory-Heidenhain stain for connective tissue. , 1950, Stain technology.

[12]  K. Endicott,et al.  Microscopic Historadiographic Technic for Locating and Quantitating Radioactive Elements in Tissues , 1947, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[13]  W. Tomlinson,et al.  A Simple Method of Staining Malaria Protozoa and Other Parasites in Paraffin Sections , 1944 .

[14]  E. Deuchar.  Staining sections before autoradiographic exposure: excessive background graining caused by Celestin blue. , 1962, Stain technology.

[15]  D. J. Bowie,et al.  Gallocyanin as a nuclear stain in autoradiography. , 1960, Stain technology.

[16]  H. Diserens,et al.  A method for radioautographic localization of isotopes in tissues. , 1952, Texas reports on biology and medicine.

[17]  P. Fitzgerald,et al.  Staining of radioautographs with metanil yellow and iron hematoxylin. , 1951, Stain technology.