Thin-layer chromatography with biological detection in phytochemistry.

Bioautography on thin-layer chromatographic (TLC) plates is a means of detecting the biological activity of a sample which has migrated on the plate with a suitable solvent. It only requires small amounts of sample and is ideal for the investigation of plant constituents, which often occur as complex mixtures. It can be used for the target-directed isolation of these constituents. In contrast to HPLC, many samples can be run at the same time on TLC. Organic solvents, which cause inactivation of enzymes or death of living organisms, can be completely removed before biological detection. Many bioassays are compatible with TLC. Antimicrobial, radical scavenging, antioxidant activities and enzyme inhibition feature among the tests that are employed.

[1]  G. Oertel,et al.  Determination of steroids by densitometry of derivatives. II. Direct fluorometry of DANSYL estrogens. , 1970, Journal of chromatography.

[2]  Dongzhu Duan,et al.  Modified TLC bioautographic method for screening acetylcholinesterase inhibitors from plant extracts. , 2009, Journal of separation science.

[3]  D. Nagle,et al.  A new 2D-TLC bioautography method for the discovery of novel antifungal agents To control plant pathogens. , 2000, Journal of natural products.

[4]  C. Weins,et al.  Toxicological evaluation of harmful substances by in situ enzymatic and biological detection in high-performance thin-layer chromatography. , 1996, Journal of chromatography. A.

[5]  R. Fischer,et al.  Zum papierchromatographischen Nachweis von Penicillinpräparaten , 1961 .

[6]  H. Mcleod,et al.  Enzymatic detection of ten organophosphorus pesticides and carbaryl on thin-layer chromatograms: an evaluation of indoxyl, substituted indoxyl and 1-naphthyl acetates as substrates of esterases. , 1968, The Analyst.

[7]  Ralf Thiericke,et al.  Drug Discovery from Nature , 2000 .

[8]  J. Espín,et al.  An easy and fast test to compare total free radical scavenger capacity of foodstuffs , 2000 .

[9]  K. Hostettmann,et al.  Naphthoquinones of Diospyros usambarensis; their Molluscicidal and Fungicidal Activities1. , 1984, Planta medica.

[10]  C. Alviano,et al.  Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants. , 2005, Journal of ethnopharmacology.

[11]  M. Recio,et al.  Screening methods for natural products with antimicrobial activity: a review of the literature. , 1988, Journal of ethnopharmacology.

[12]  K. Courtney,et al.  A new and rapid colorimetric determination of acetylcholinesterase activity. , 1961, Biochemical pharmacology.

[13]  K. Hostettmann,et al.  Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents , 2003 .

[14]  F. Dayan,et al.  Activity of quinones on colletotrichum species. , 2003, Journal of agricultural and food chemistry.

[15]  Tomasz Mroczek,et al.  Pressurized liquid extraction and anticholinesterase activity-based thin-layer chromatography with bioautography of Amaryllidaceae alkaloids. , 2009, Analytica chimica acta.

[16]  G. Lyon,et al.  Detection of inhibitions of Erwinia carotovora and E. herbicola on thin-layer chromatogram , 1975 .

[17]  B. Kocsis,et al.  A Sensitive Method for Thin-Layer Chromatographic Detection of Amphotericin B , 2010, JPC – Journal of Planar Chromatography – Modern TLC.

[18]  R. Verpoorte,et al.  Qualitative determination of false-positive effects in the acetylcholinesterase assay using thin layer chromatography. , 2003, Phytochemical analysis : PCA.

[19]  G. Cordell,et al.  A direct bioautographic tlc assay for compounds possessing antibacterial activity. , 1987, Journal of natural products.

[20]  W. Gritsanapan,et al.  In vitro antigonococcal activity of Coscinium fenestratum stem extract. , 2009, Journal of ethnopharmacology.

[21]  Kurt Hostettmann,et al.  A bioautographic agar overlay method for the detection of antifungal compounds from higher plants , 1991 .

[22]  B. Kocsis,et al.  The combination of the overpressured layer chromatography and bioautography and its applications to the analysis of molecules influencing cell proliferation , 2003 .

[23]  A. Urbain,et al.  Natural Product Inhibitors of Acetylcholinesterase , 2006 .

[24]  E. E. Miller,et al.  Cottonseed flavonoids as lipid antioxidants , 1984 .

[25]  B. Kocsis,et al.  Optimization of bioassay method for the quantitative microbiological determination of amphotericin B , 2010, Mycoses.

[26]  E. E. Miller,et al.  A flavonoid antioxidant in Spanish peanuts(Arachia hypogoea) , 1984 .

[27]  Colin F. Poole,et al.  Encyclopedia of analytical science , 1995 .

[28]  R. Hancock,et al.  Use of specific dyes in the detection of antimicrobial compounds from crude plant extracts using a thin layer chromatography agar overlay technique , 1995 .

[29]  K. Hostettmann,et al.  Bio‐guided isolation of cholinesterase inhibitors from the bulbs of , 2005, Phytotherapy research : PTR.

[30]  K. Hostettmann,et al.  A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts. , 2009, Phytochemical analysis : PCA.

[31]  Melissa Hanna-Brown,et al.  Application of retention modelling to the simulation of separation of organic anions in suppressed ion chromatography. , 2009, Journal of chromatography. A.

[32]  T. Mroczek.  Highly efficient, selective and sensitive molecular screening of acetylcholinesterase inhibitors of natural origin by solid-phase extraction-liquid chromatography/electrospray ionisation-octopole-orthogonal acceleration time-of-flight-mass spectrometry and novel thin-layer chromatography-based bioau , 2009, Journal of chromatography. A.

[33]  R. Furlan,et al.  A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers. , 2006, Phytochemical analysis : PCA.

[34]  A. Longland,et al.  A convenient, sensitive and rapid assay for antibacterial activity of phytoalexins , 1989 .

[35]  Philippe N. Okusa,et al.  Optimization of the Culture Medium Used for Direct TLC-Bioautography. Application to the Detection of Antimicrobial Compounds from Cordia gilletii De Wild (Boraginaceae) , 2010, JPC – Journal of Planar Chromatography – Modern TLC.

[36]  K. Ingkaninan,et al.  Screening for acetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining. , 2001, Journal of chromatography. A.

[37]  G. Morlock,et al.  Hyphenations in planar chromatography. , 2010, Journal of chromatography. A.

[38]  S. Rankin,et al.  Bioautography and chemical characterization of antimicrobial compound(s) in commercial water-soluble annatto extracts. , 2005, Journal of agricultural and food chemistry.

[39]  C. Rice-Evans,et al.  Factors influencing the antioxidant activity determined by the ABTS.+ radical cation assay. , 1997, Free radical research.

[40]  H. Kurihara,et al.  A New Screening Method for Glucosidase Inhibitors and its Application to Algal Extracts , 1994 .

[41]  B. Walker,et al.  Inhibition of human immunodeficiency virus syncytium formation and virus replication by castanospermine. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[42]  R. Furlan,et al.  A rapid TLC autographic method for the detection of glucosidase inhibitors. , 2007, Phytochemical analysis : PCA.

[43]  W. J. Begue,et al.  The use of tetrazolium salts in bioautographic procedures. , 1972, Journal of chromatography.

[44]  P. Houghton.  Use of small scale bioassays in the discovery of novel drugs from natural sources , 2000, Phytotherapy research : PTR.

[45]  Nicole Zitzmann,et al.  α‐Glucosidase inhibitors as potential broad based anti‐viral agents , 1998, FEBS letters.

[46]  J. Nuss,et al.  Current therapies and emerging targets for the treatment of diabetes. , 2001, Current pharmaceutical design.

[47]  M. Choudhary,et al.  Inhibition of α-glucosidase by oleanolic acid and its synthetic derivatives , 2002 .

[48]  P. Ott,et al.  Investigation of Chelidonium Alkaloids by Use of a Complex Bioautographic System , 2006, JPC – Journal of Planar Chromatography – Modern TLC.

[49]  M. Mazurek,et al.  Chromatographic analysis of chemical warfare agents. , 1990, Journal of chromatography.

[50]  A. A. Levi,et al.  A Microchromatographic Method for the Detection and Approximate Determination of the Different Penicillins in a Mixture , 1946, Nature.

[51]  A L Homans,et al.  Direct bioautography on thin-layer chromatograms as a method for detecting fungitoxic substances. , 1970, Journal of chromatography.

[52]  K. Asperen.  A study of housefly esterases by means of a sensitive colorimetric method , 1962 .

[53]  Klaus Burger,et al.  Bioactivity screening by chromatography-bioluminescence coupling , 1996 .

[54]  Lars Bohlin,et al.  Bioassay methods in natural product research and drug development : proceedings of the international symposium on Bioassay Methods in Natural Product Research and Drug Development, held at the Biomedical Centre of Uppsala University, Sweden, from 24-27 August 1997 , 1999 .

[55]  V. Betina.  Bioautography in paper and thin-layer chromatography and its scope in the antibiotic field. , 1973, Journal of chromatography.

[56]  A Marston,et al.  A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants. , 2002, Phytochemical analysis : PCA.

[57]  M. S. Blois,et al.  Antioxidant Determinations by the Use of a Stable Free Radical , 1958, Nature.

[58]  K. Hostettmann,et al.  Search for new antifungal compounds from higher plants , 1994 .

[59]  N. Watanabe,et al.  A Simple Screening Method for Antioxidants and Isolation of Several Antioxidants Produced by Marine Bacteria from Fish and Shellfish , 1994 .

[60]  P. Carrupt,et al.  In vitro screening assays to identify natural or synthetic acetylcholinesterase inhibitors: thin layer chromatography versus microplate methods. , 2008, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[61]  E. Loveman,et al.  A systematic review of the clinical effectiveness of donepezil, rivastigmine and galantamine on cognition, quality of life and adverse events in Alzheimer's disease , 2006, International journal of geriatric psychiatry.

[62]  R. Gutiérrez,et al.  High-performance thin-layer chromatography-bioautography for multiple antibiotic residues in cow's milk. , 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.