Ion-Exchange Membrane Chromatography as an Alternative Method of Separation of Potato y Virus

Abstract Procedures of separation of virus particles from a plant material are multistage. Furthermore often they are difficult in terms of methodology and require use of expensive, highly specialist equipment and yield of separation is often low. The antigen obtained is often degraded and contains admixtures of other proteins. Therefore, generation of high quality and specificity antibodies based on such antigen is very difficult and quality of the antibodies has impact on reliability, sensitivity and unambiguity of results of immunodiagnostic tests (e.g. ELISA) that are currently conventionally used to detect vegetable viruses. In this study three conventionally-performed methods of separation of potato virus Y (PVY) were compared and a method of separation based on membrane chromatography, as an alternative separation technique, has been presented. It has been demonstrated that in proper process conditions good quality virus preparation can be obtained.

[1]  S. Wróbel Modification of ELISA by Replacing Incubation of Microtiter Plates in an Incubator with Their Shaking in PVY, PVM and PLRV Detection , 2014, American Journal of Potato Research.

[2]  J. Schubert,et al.  Comparison of three methods for the detection of Potato virus Y in seed potato certification , 2013 .

[3]  I. Gutiérrez-Aguirre,et al.  Fast purification of the filamentous Potato virus Y using monolithic chromatographic supports. , 2013, Journal of chromatography. A.

[4]  A. Schultz,et al.  A novel method for concentrating hepatitis A virus and caliciviruses from bottled water. , 2009, Journal of virological methods.

[5]  P. Alves,et al.  Anion-exchange membrane chromatography for purification of rotavirus-like particles , 2008 .

[6]  A. Podgornik,et al.  Purification and concentration of bacteriophage T4 using monolithic chromatographic supports. , 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[7]  J. Valkonen Viruses: Economical Losses and Biotechnological Potential , 2007 .

[8]  Uwe Gottschalk,et al.  New Q membrane scale-down model for process-scale antibody purification. , 2006, Journal of chromatography. A.

[9]  M. Chrzanowska New isolates of the necrotic strain of potato virus Y (PVYN) found recently in Poland , 1991, Potato Research.

[10]  Joe X. Zhou,et al.  Basic Concepts in Q Membrane Chromatography for Large‐Scale Antibody Production , 2006, Biotechnology progress.

[11]  R. Singh,et al.  Possible Escape of a Recombinant Isolate of Potato virus Y by Serological Indexing and Methods of its Detection. , 2003, Plant disease.

[12]  S. German-Retana,et al.  Interaction between potyvirus helper component-proteinase and capsid protein in infected plants. , 2002, The Journal of general virology.

[13]  Raja Ghosh,et al.  Protein separation using membrane chromatography: opportunities and challenges. , 2002, Journal of chromatography. A.

[14]  M. Shields,et al.  Plasmid purification using membrane-based anion-exchange chromatography. , 2001, Analytical biochemistry.

[15]  H. Granzow,et al.  Simple and rapid purification of alphaherpesviruses by chromatography on a cation exchange membrane. , 1998, Journal of virological methods.

[16]  N. Cerovska,et al.  Optimization of purification procedure for potato virus Y strain NN. , 1997, Acta virologica.

[17]  R. Tuma,et al.  Purification of viruses and macromolecular assemblies for structural investigations using a novel ion exchange method. , 1994, Virology.

[18]  J. Bernstein,et al.  Active respiratory syncytial virus purified by ion-exchange chromatography: characterization of binding and elution requirements. , 1992, Journal of virological methods.

[19]  R. Lawson,et al.  An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissue. , 1988, Journal of virological methods.

[20]  R E Rhoads,et al.  Purification and characterization of potyvirus helper component. , 1985, Virology.

[21]  A. N. Adams,et al.  Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. , 1977, The Journal of general virology.

[22]  H. Paul SDS Polyacrylamide Gel Electrophoresis of Virion Proteins as a Tool for detecting the Presence of Virus in Plants.: II. Examination of further Virus-Host Combinations , 1975 .

[23]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[24]  N. I. Blagoveshchenskii,et al.  The control of Potato virus diseases. , 1970 .