Multiple auxin response modules in the soybean SAUR 15A promoter

Abstract The soybean SAUR genes are primary auxin response genes that are induced within a few minutes after auxin application. It has been previously shown that a small region (referred to as the DUE/NDE element) in the SAUR 15A promoter is sufficient to confer auxin responsiveness [Li et al., Plant Physiol 106 (1994) 37–43]. Here, we describe a second auxin response region of 47-bp in the SAUR 15A promoter that resides upstream of the DUE/NDE element. We also report on site-directed mutations in the NDE/DUE element that suggest a complex organization for this auxin-response element. Our results indicate that the SAUR 15A promoter is composed of multiple auxin response elements that behave differently than those previously characterized in the soybean GH3 promoter [Liu et al. Plant Cell, 6 (1994) 645–657].

[1]  G. Hagen,et al.  Tissue-specific and organ-specific expression of soybean auxin-responsive transcripts GH3 and SAURs. , 1991, The Plant cell.

[2]  P. Oeller,et al.  Structural characterization of the early indoleacetic acid-inducible genes, PS-IAA4/5 and PS-IAA6, of pea (Pisum sativum L.). , 1993, Journal of molecular biology.

[3]  A. Theologis,et al.  Identification of the auxin-responsive element, AuxRE, in the primary indoleacetic acid-inducible gene, PS-IAA4/5, of pea (Pisum sativum). , 1993, Journal of molecular biology.

[5]  A. Theologis,et al.  Early Genes and Auxin Action , 1996, Plant physiology.

[6]  T. Guilfoyle,et al.  Rapid redistribution of auxin-regulated RNAs during gravitropism. , 1989, Science.

[7]  A. Theologis,et al.  DNA elements responsive to auxin , 1996, BioEssays : news and reviews in molecular, cellular and developmental biology.

[8]  A. Theologis,et al.  Two auxin-responsive domains interact positively to induce expression of the early indoleacetic acid-inducible gene PS-IAA4/5. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[9]  R. Napier,et al.  Auxin action and auxin-binding proteins. , 1995, The New phytologist.

[10]  A. Theologis,et al.  Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. , 1994, The Plant journal : for cell and molecular biology.

[11]  G. Hagen,et al.  Soybean GH3 promoter contains multiple auxin-inducible elements. , 1994, The Plant cell.

[12]  G. Hagen,et al.  An Auxin-Inducible Element in Soybean SAUR Promoters , 1994, Plant physiology.

[13]  J. Ellis,et al.  In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants , 1993 .

[14]  G. Hagen,et al.  Composite structure of auxin response elements. , 1995, The Plant cell.