Removal of promoter nucleosomes by disassembly rather than sliding in vivo.

Previous work demonstrated the removal of nucleosomes from the PHO5 promoter upon transcriptional activation in yeast. Removal could occur by nucleosome disassembly or by sliding of nucleosomes away from the promoter. We have now activated the PHO5 promoter on chromatin circles following excision from the chromosomal locus. Whereas sliding would conserve the number of nucleosomes on the circle, we found that the number was diminished, demonstrating chromatin remodeling by nucleosome disassembly.

[1]  J. Mellor,et al.  In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p. , 2001, Genes & development.

[2]  M. Ptashne,et al.  RNA Polymerase II Holoenzyme Recruitment Is Sufficient to Remodel Chromatin at the Yeast PHO5 Promoter , 1997, Cell.

[3]  F. Thoma,et al.  Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: A model system for study of higher order structure , 1985, Cell.

[4]  J Seth Strattan,et al.  Nucleosomes unfold completely at a transcriptionally active promoter. , 2003, Molecular cell.

[5]  R. Kornberg,et al.  RSC unravels the nucleosome. , 2001, Molecular cell.

[6]  Andrew Flaus,et al.  Nucleosome mobilization catalysed by the yeast SWI/SNF complex , 1999, Nature.

[7]  E. O’Shea,et al.  Regulation of Chromatin Remodeling by Inositol Polyphosphates , 2002, Science.

[8]  J. Wang,et al.  Conformational fluctuations of DNA helix. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[9]  B. Révet,et al.  Chromatin reconstitution on small DNA rings. II. DNA supercoiling on the nucleosome. , 1988, Journal of molecular biology.

[10]  H. Reinke,et al.  Multiple Mechanistically Distinct Functions of SAGA at the PHO5 Promoter , 2003, Molecular and Cellular Biology.

[11]  N. Cozzarelli,et al.  Helical repeat and linking number of surface-wrapped DNA. , 1988, Science.

[12]  G. Längst,et al.  Nucleosome Movement by CHRAC and ISWI without Disruption or trans-Displacement of the Histone Octamer , 1999, Cell.

[13]  Dimitris Thanos,et al.  Nucleosome Sliding via TBP DNA Binding In Vivo , 2001, Cell.

[14]  Ali Hamiche,et al.  ATP-Dependent Histone Octamer Sliding Mediated by the Chromatin Remodeling Complex NURF , 1999, Cell.

[15]  E. O’Shea,et al.  Signaling phosphate starvation. , 1996, Trends in biochemical sciences.

[16]  H. Reinke,et al.  Histones are first hyperacetylated and then lose contact with the activated PHO5 promoter. , 2003, Molecular cell.

[17]  R. Kornberg,et al.  Affinity Purification of Specific Chromatin Segments from Chromosomal Loci in Yeast , 2003, Molecular and Cellular Biology.

[18]  T. Tsukiyama,et al.  Chromatin remodeling in vivo: evidence for a nucleosome sliding mechanism. , 2003, Molecular cell.

[19]  J. Vinograd,et al.  The number of superhelical turns in native Virion SV40 DNA and Minicol DNA determined by the band counting method , 1976, Cell.

[20]  W. Keller.  Characterization of purified DNA-relaxing enzyme from human tissue culture cells. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[21]  K. Fascher,et al.  Nucleosome disruption at the yeast PHO5 promoter upon PHO5 induction occurs in the absence of DNA replication , 1992, Cell.

[22]  P. Chambon,et al.  Folding of the DNA double helix in chromatin-like structures from simian virus 40. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[23]  W Hörz,et al.  Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast. , 1986, The EMBO journal.

[24]  R. Kingston,et al.  Functional Differences between the Human ATP-dependent Nucleosome Remodeling Proteins BRG1 and SNF2H* , 2001, The Journal of Biological Chemistry.

[25]  C. Peterson,et al.  SWI-SNF-Mediated Nucleosome Remodeling: Role of Histone Octamer Mobility in the Persistence of the Remodeled State , 2000, Molecular and Cellular Biology.

[26]  S. Henikoff,et al.  The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. , 2002, Molecular cell.

[27]  Carl Wu,et al.  Purification and properties of an ATP-dependent nucleosome remodeling factor , 1995, Cell.

[28]  Michael R. Green,et al.  Nucleosome disruption and enhancement of activator binding by a human SW1/SNF complex , 1994, Nature.

[29]  S. Berger,et al.  Absence of Gcn5 HAT activity defines a novel state in the opening of chromatin at the PHO5 promoter in yeast. , 1998, Molecular cell.