Exploiting bacteriophage proteomes : the hidden biotechnological 1 potential 2 3

17 Bacteriophages encode many distinct proteins for the successful infection of a 18 bacterial host. Each protein plays a specific role in the phage replication cycle, from 19 host recognition, through takeover of the host machinery, and up to cell lysis for 20 progeny release. As the roles of these proteins are being revealed, more 21 biotechnological applications can be anticipated. Phage-encoded proteins are now 22 being explored for the control, detection and typing of bacteria; as vehicles for drug 23 delivery; and for vaccine development. In this review we discuss how engineering 24 approaches can be used to improve the natural properties of these proteins, and set 25 forth the most innovative applications that demonstrate the unlimited 26 biotechnological potential held by phage-encoded proteins. 27 28

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