Heterogeneity of 5‐hydroxytryptamine receptors in the rat uterus and stomach strip

1 Experiments were performed using the rat isolated uterus and the rat stomach strip to investigate the effects of 5‐hydroxytryptamine (5‐HT) on 5‐HT receptors in the presence of different antagonists. Amitriptyline, methysergide and trazodone were used to antagonize the response to 5‐HT in these tissues. 2 The pA2 value for amitriptyline with 5‐HT as the agonist was estimated from the Schild plot analysis and found to differ significantly (P<0.05) in the stomach strip (6.36) from that in the isolated uterus (9.06). 3 A similar difference was found using trazodone; here the pA2 value was 6.74 in the stomach strip and 8.49 in the isolated uterus. 4 These results indicate a difference between the two tissues. This difference is discussed in terms of heterogeneity of 5‐HT receptors.

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