Human beta-defensin 2 promotes the proliferation of lung cancer cells through ATP-binding cassette transporter G 2

Objective: The aim of this study was to investigate the effect of human beta-defensin 2 (hBD2) and ATPbinding cassette transporter G2 (ABCG2) on the proliferation of lung cancer cells. Methods: One hundred and thirty patients with non-small cell lung cancer were recruited. All the patients underwent pulmonary lobectomy and the specimens (lung cancer tissues and corresponding non-tumor normal tissues of 130 patients) were collected. All the specimens were divided into two groups: NSCLC group (lung cancer tissues) and Control group (corresponding non-tumor normal tissues). Protein HBD2 and ABCG2 expressions were detected by immunohistochemistry. An in vitro study was also performed using A549 cells. A549 cells were treated with hBD2, proliferation was analyzed using MTT and ABCG2 expression was detected by western blotting. Next, A549 cells were pretreated with Fumitremorgin C, a selective ABCG2 inhibitor, and cell proliferation and ABCG2 expression were investigated. Results: Protein hBD2 and ABCG2 expressions in the NSCLC group were both higher compared with the control group (both P<0.01). Furthermore, hBD2 induced ABCG2 expression and A549 proliferation in a doseand time-dependent manner (both P<0.01), while these effects were inhibited by the Fumitremorgin C treatment (both P<0.01). Conclusion: hBD2 induced the proliferation of lung cancer cells through ABCG2, thus playing an important role in the development of NSCLC.

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