Dodecylsulfate-coated monolithic octadecyl-bonded silica stationary phase for high-speed separation of hydrogen, magnesium and calcium in rainwater.

The high-speed determination of hydrogen, magnesium and calcium ions by ion chromatography (IC) is demonstrated on a monolithic octadecyl-boned silica (ODS) column coated with lithium dodecylsulfate (Li-DS). This stationary phase, when used in conjunction with a 2 mM ethylenediamine and 0.1 mM Li-DS solution as eluent at pH 6.0, was found to be suitable for the rapid and efficient separation of hydrogen and magnesium and calcium in the order H+ < Mg2+ < Ca2+ within 4 min at a flow rate of 4.0 ml/min. Under the conditions, linear calibration plots of conductivity versus concentration were obtained for the cations over about three orders of magnitude, and the detection limits were 1 microM for H+, 2 microM for Mg2+ and Ca2+. Rainwater was analyzed directly using this IC system with satisfactory results.

[1]  J. Kirkland,et al.  Superficially porous silica microspheres for fast high-performance liquid chromatography of macromolecules. , 2000, Journal of chromatography. A.

[2]  S. Hjertén,et al.  High-performance liquid chromatography on continuous polymer beds , 1989 .

[3]  K. Nakanishi,et al.  A New Monolithic‐Type HPLC Column For Fast Separations , 2000 .

[4]  M. Mori,et al.  Monolithic ODS-silica gel column for determination of hydrogen, sodium, ammonium and potassium in acid rain by lon chromatography , 2003 .

[5]  M. Nagai,et al.  Ion chromatographic analysis of hydronium ion using ODS modified with dodecylsulfate as the stationary phase. , 2000, The Analyst.

[6]  Joseph Wang,et al.  Solid-state pH nanoelectrode based on polyaniline thin film electrodeposited onto ion-beam etched carbon fiber , 2002 .

[7]  Yang Li,et al.  Continuous beds: high-resolving, cost-effective chromatographic matrices , 1992, Nature.

[8]  P. Hajós.  Ethylenediamine as eluent component in cation chromatography. Predictive and comparative study for analysis of alkaline earth ions. , 2002, Journal of chromatography. A.

[9]  T. Yagi,et al.  Table of isotachophoretic indices : I. Simulated qualitative and quantitative indices of 287 anionic substances in the range ph 3–10 , 1983 .

[10]  P. Haddad,et al.  Rapid and direct determination of iodide in seawater by electrostatic ion chromatography. , 2002, Journal of chromatography. A.

[11]  C. Lucy,et al.  Ultra-fast HPLC separation of common anions using a monolithic stationary phase. , 2002, The Analyst.

[12]  L. Terenius,et al.  Continuous beds for microchromatography: cation-exchange chromatography. , 1994, Analytical biochemistry.

[13]  B. Paull,et al.  Fast ion chromatography of common inorganic anions on a short ODS column permanently coated with didodecyldimethylammonium bromide. , 2002, Journal of chromatography. A.

[14]  C. Kinchin,et al.  Ion chromatographic determination of acidity. , 2000, Analytical chemistry.

[15]  J. Fritz,et al.  Determination of total acidity and of divalent cations by ion chromatography with n-hexadecylphosphocholine as the stationary phase. , 2002, Journal of chromatography. A.

[16]  A. Iles,et al.  High-resolution determination of H+ by ion chromatography. Application to the simultaneous determination of H+, Na+, NH4+ and K+ in acid rain. , 2001, The Analyst.

[17]  J. Henion,et al.  Fast liquid chromatographic-mass spectrometric determination of pharmaceutical compounds. , 1999, Journal of chromatography. B, Biomedical sciences and applications.

[18]  K. Nakanishi,et al.  Octadecylsilylated porous silica rods as separation media for reversed-phase liquid chromatography. , 1996, Analytical chemistry.

[19]  R. Plumb,et al.  Use of monolithic silica columns to increase analytical throughput for metabolite identification by liquid chromatography/tandem mass spectrometry , 2001 .

[20]  R. Vreeken,et al.  Rapid target analysis of microcontaminants in water by on-line single-short-column liquid chromatography combined with atmospheric pressure chemical ionization tandem mass spectrometry. , 1997, Journal of chromatography. A.

[21]  S. Hjertén,et al.  Hydrophobic-interaction chromatography of proteins on continuous beds derivatized with isopropyl groups , 1996 .

[22]  John G. Dorsey,et al.  Equations for calculation of chromatographic figures of merit for ideal and skewed peaks , 1983 .

[23]  Wenzhi Hu,et al.  Monolithic octadecylsilyl-silica gel column for the high-speed ion chromatographic determination of acidity. , 2003, Journal of chromatography. A.