In vitro Shoot Regeneration of Bacopa monnieri (L.) Using Cyanobacterial Media- A Novel Approach and Effect of Phytoregulators on in vitro micropropagation

This review highlights the recent development and n ovel achievements made for the multiple shoots rege neration of Bacopa monnieri in Cyanobacterial medium as well as in MS medium. Shootlets were regenerated from noda l explants of stem through auxiliary shoot proliferat ion. In Cyanobacterial culture medium Bacopa monnie ri survived for long period of time and multiple shoot initiati on was observed in 2mg/l Kn. When Bacopa monnieri w as inoculated in MS medium supplemented with different concentrations of PGR’s, maximum no. of shoots wer e observed in 0.5 mg/l BAP + 2.0 mg/l Kn and 0.5 mg/ l Kn+1.0 mg/l BAP.