TAL effector-DNA specificity

TAL effectors are important virulence factors of bacterial plant pathogenic Xanthomonas, which infect a wide variety of plants including valuable crops like pepper, rice, and citrus. TAL proteins are translocated via the bacterial type III secretion system into host cells and induce transcription of plant genes by binding to target gene promoters. Members of the TAL effector family differ mainly in their central domain of tandemly arranged repeats of typically 34 amino acids each with hypervariable di-amino acids at positions 12 and 13. We recently showed that target DNA-recognition specificity of TAL effectors is encoded in a modular and clearly predictable mode. The repeats of TAL effectors feature a surprising one repeat-to-one-bp correlation with different repeat types exhibiting a different DNA base pair specificity. Accordingly, we predicted DNA specificities of TAL effectors and generated artificial TAL proteins with novel DNA recognition specificities. We describe here novel artificial TALs and discuss implications for the DNA recognition specificity. The unique TAL-DNA binding domain allows design of proteins with potentially any given DNA recognition specificity enabling many uses for biotechnology.

[1]  G. Van den Ackerveken,et al.  Eukaryotic features of the Xanthomonas type III effector AvrBs3: protein domains involved in transcriptional activation and the interaction with nuclear import receptors from pepper. , 2001, The Plant journal : for cell and molecular biology.

[2]  Jens Boch,et al.  Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors , 2009, Science.

[3]  U. Bonas,et al.  Xanthomonas AvrBs3 family-type III effectors: discovery and function. , 2010, Annual review of phytopathology.

[4]  T. Lahaye,et al.  A single plant resistance gene promoter engineered to recognize multiple TAL effectors from disparate pathogens , 2009, Proceedings of the National Academy of Sciences.

[5]  Simone Hahn,et al.  A Bacterial Effector Acts as a Plant Transcription Factor and Induces a Cell Size Regulator , 2007, Science.

[6]  Jens Boch,et al.  Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae. , 2010, The New phytologist.

[7]  U. Bonas,et al.  Detailed analysis of the DNA recognition motifs of the Xanthomonas type III effectors AvrBs3 and AvrBs3Deltarep16. , 2009, The Plant journal : for cell and molecular biology.

[8]  Jan Grau,et al.  Recognition of AvrBs 3-Like Proteins Is Mediated by Specific Binding to Promoters of Matching Pepper Bs 3 Alleles 1 [ W ] , 2009 .

[9]  Matthew J. Moscou,et al.  A Simple Cipher Governs DNA Recognition by TAL Effectors , 2009, Science.

[10]  S. He,et al.  Type III Protein Secretion in Plant Pathogenic Bacteria , 2009, Plant Physiology.

[11]  W. Zhu,et al.  AvrXa10 contains an acidic transcriptional activation domain in the functionally conserved C terminus. , 1998, Molecular plant-microbe interactions : MPMI.

[12]  Sebastian Schornack,et al.  Gene-for-gene-mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins. , 2006, Journal of plant physiology.

[13]  G. Van den Ackerveken,et al.  The xanthomonas type III effector protein AvrBs3 modulates plant gene expression and induces cell hypertrophy in the susceptible host. , 2002, Molecular plant-microbe interactions : MPMI.

[14]  Jan Grau,et al.  Recognition of AvrBs3-Like Proteins Is Mediated by Specific Binding to Promoters of Matching Pepper Bs3 Alleles1[W] , 2009, Plant Physiology.

[15]  Simone Hahn,et al.  Plant Pathogen Recognition Mediated by Promoter Activation of the Pepper Bs3 Resistance Gene , 2007, Science.