Evaluation of Immunoglobulin G Subclass Antibodies against Recombinant Fasciola gigantica Cathepsin L1 in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Human Fasciolosis

ABSTRACT A cystatin capture enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola gigantica cathepsin L1 antigen was developed to detect specific immunoglobulin G (IgG) subclass antibodies (IgG1, IgG2, IgG3, and IgG4) and was evaluated for its diagnostic potential for human fasciolosis. In an analysis of the sera of 13 patients infected with F. gigantica, 209 patients with other parasitic infections, 32 cholangiocarcinoma patients, and 42 healthy controls, the IgG4-ELISA gave the highest diagnostic values. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method based on the detection of IgG4 antibody were 100%, 99.3%, 99.3%, 86.7%, and 100%, respectively. The results revealed that restricting the ELISA to the detection of specific IgG4 antibody enhanced the specificity and accuracy for the serodiagnosis of human fasciolosis.

[1]  C. Wongkham,et al.  Serodiagnosis of human fascioliasis by a cystatin capture enzyme-linked immunosorbent assay with recombinant Fasciola gigantica cathepsin L antigen. , 2005, The American journal of tropical medicine and hygiene.

[2]  Gerald B. Pier,et al.  Immunology, Infection, and Immunity , 2004 .

[3]  J. Allen,et al.  Immunity to Parasitic and Fungal Infections , 2004 .

[4]  C. Wongkham,et al.  Immunodiagnosis of human fascioliasis using an antigen of Fasciola gigantica adult worm with the molecular mass of 27 kDa by a dot-ELISA. , 2003, The Southeast Asian journal of tropical medicine and public health.

[5]  M. Rokni,et al.  Comparison of adult somatic and cysteine proteinase antigens of Fasciola gigantica in enzyme linked immunosorbent assay for serodiagnosis of human fasciolosis. , 2003, Acta tropica.

[6]  T. Nutman,et al.  Class and subclass selection in parasite-specific antibody responses. , 2003, Trends in parasitology.

[7]  P. Chiodini,et al.  Gnathostomiasis: An Emerging Imported Disease , 2003, Emerging Infectious Diseases.

[8]  K. Sawanyawisuth,et al.  Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis , 2003, Parasitology Research.

[9]  M. Rokni,et al.  Diagnosis of human fasciolosis in the Gilan province of Northern Iran: application of cathepsin L-ELISA. , 2002, Diagnostic microbiology and infectious disease.

[10]  A. Saleh,et al.  Dot ELISA for measuring anti-Fasciola IgG isotypes among patients with fascioliasis. , 2002, Journal of the Egyptian Society of Parasitology.

[11]  C. Carmona,et al.  Immunodiagnosis of fasciolosis using recombinant procathepsin L cystein proteinase. , 2001, Diagnostic microbiology and infectious disease.

[12]  J. Cornelissen,et al.  Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease. , 2001, International journal for parasitology.

[13]  J. McCarthy,et al.  Emerging helminth zoonoses. , 2000, International journal for parasitology.

[14]  M. Hassan,et al.  Anti-fasciola IgG isotypes among patients with fascioliasis before and after treatment. , 2000, Journal of the Egyptian Society of Parasitology.

[15]  S. H. Lee,et al.  Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis. , 1999, The Korean journal of parasitology.

[16]  C. Wongkham,et al.  Fasciola gigantica-specific antigens: purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis. , 1999, The American journal of tropical medicine and hygiene.

[17]  J. Dalton,et al.  Short report: Diagnosis of human fascioliasis: detection of anti-cathepsin L antibodies in blood samples collected on filter paper. , 1999, The American journal of tropical medicine and hygiene.

[18]  J. Cornelissen,et al.  Use of a pre-selected epitope of cathepsin-L1 in a highly specific peptide-based immunoassay for the diagnosis of Fasciola hepatica infections in cattle. , 1999, International journal for parasitology.

[19]  J. Dalton,et al.  Short report: Immunodiagnosis of human fascioliasis using recombinant Fasciola hepatica cathepsin L1 cysteine proteinase. , 1999, The American journal of tropical medicine and hygiene.

[20]  J. Dalton,et al.  IMMUNODIAGNOSIS OF HUMAN FASCIOLOSIS USING RECOMBINANT FASCIOLA HEPATICA CATHEPSIN L1 CYSTEINE PROTEINASE , 1999 .

[21]  S. Andrews,et al.  The Life Cycle of Fasciola hepatica , 1999 .

[22]  J. Dalton,et al.  Immunodiagnosis of Fasciola hepatica infection (fascioliasis) in a human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase. , 1998, The American journal of tropical medicine and hygiene.

[23]  C. Wongkham,et al.  Comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with Fasciola gigantica. , 1996, The Southeast Asian journal of tropical medicine and public health.

[24]  A. Loukas,et al.  Immunoglobulin G subclass antibodies against excretory/secretory antigens of Ancylostoma caninum in human enteric infections. , 1996, The American journal of tropical medicine and hygiene.

[25]  R. Maizels,et al.  T-cell activation and the balance of antibody isotypes in human lymphatic filariasis. , 1995, Parasitology today.

[26]  T. Egwang,et al.  Elevated antifilarial IgG4 antibody levels in microfilaremic and microfilaridermic Gabonese adults and children. , 1993, The American journal of tropical medicine and hygiene.

[27]  S. Chanteau,et al.  Diagnostic values of ELISA-IgG4 as compared to ELISA-IgG and indirect immunofluorescence, for the routine diagnosis of bancroftian filariasis in the South Pacific. Application on capillary blood collected on filter paper. , 1991, Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit.

[28]  D. Bundy,et al.  Humoral immune responses in human infection with the whipworm Trichuris trichiura , 1991, Parasite immunology.

[29]  A. Simpson,et al.  Human IgE, IgG4 and resistance to reinfection with Schistosomahaematobium , 1991, Nature.

[30]  R. Genta,et al.  Prominence of IgG4 antibodies in the human responses to Strongyloides stercoralis infection. , 1989, The Journal of infectious diseases.

[31]  A. Jassim,et al.  Antibody isotypes in human schistosomiasis mansoni , 1987, Parasite immunology.

[32]  E. Ottesen,et al.  Prominence of IgG4 in the IgG antibody response to human filariasis. , 1985, Journal of immunology.

[33]  R. Aalberse,et al.  Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response. , 1983, Journal of immunology.

[34]  D. Erdman.  Clinical comparison of ethyl acetate and diethyl ether in the formalin-ether sedimentation technique , 1981, Journal of clinical microbiology.

[35]  P. Das,et al.  IgG4 antibodies in Egyptian patients with schistosomiasis. , 1981, International archives of allergy and applied immunology.

[36]  R. Galen,et al.  Predictive value and efficiency of laboratory testing. , 1980, Pediatric clinics of North America.

[37]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.