Maize (Zea mays L.) is one of the most important cereal crops. and one of the most significant renewable raw materials for the production of energy and many different products. Bioethanol is a biofuel that is mostly used as a replacement for fossil fuels worldwide. Major byproduct that arises from the fermentation process of corn is dried distillers' grains (DDG). Due to its high feeding value it represents an excellent component for livestock feed mixtures. The main goal of this study was to determine the technological quality of maize grain and the influence of maize hybrid on bioethanol yield and quality of this process' by-product, dried distillers' grains. Five maize hybrids developed at the Maize Research Institute, Zemun Polje, were investigated in this study. The lowest concentration of bioethanol in the fermentation medium after 44h of alcoholic fermentation (8.64% w/w) was obtained by hybrid ZP 560, and the highest (9.10% w/w) by hybrid ZP 600, while compared to the maximum theoretical bioethanol yield, the highest scoring hybrid was ZP 606 (93.59%) and the lowest ZP 505 (87.33%). The highest in vitro dry matter digestibility of DDG was determined in a sample obtained from hybrid ZP 505 (82.41%) and the lowest (77.12%) in the DDG sample of ZP 606. The chemical composition and physical characteristics of the grain, as well as other parameters of the production process have influenced the overall bioethanol yield. Hybrids created in the Maize Research Institute represent unique starting material for research of the possibilities of bioethanol, starch, food and animal feed production.