Biopulping studies using an effluent isolate Cuwularia lunata LW6

The lignin is unwanted constituent for a pulp and paper manufacturer, which must be removed for getting good quality paper. The aim of bio-delignification of raw material is to make pulping process easier, to save energy requirements, to improve pulp properties and to minimize pollution. The present study aims to find suitability of isolate Curvularia lunata LW6 for biopulping of sugarcane bagasse. The organism was screened from effluent of pulp and paper mill and rested for lignolytic ability. The isolate produced the enzyme lignin peroxidase (3.31 U/mg of protein) and laccase (6.12 UImL). It showed good saprophytic ability (38.14 cm2) on sterile bagasse when supplemented with glucose and yeast extract as cosubstrate. The solid-state fermentation of bagasse for biopulping resulted in loss of weight by 21.66%, while lignin content and kappa number decreased by 44.05% and 40.78%, respectively. The chemical pulping showed 55.25% reduction in kappa number; however, it required high temperature, pressure and alkaline medium. The mutagenesis studies showed slight decrease in cellulase activity of the isola_te.

[1]  K. Eriksson,et al.  Biotechnology in the pulp and paper industry , 1990, Wood Science and Technology.

[2]  H. Kern.  Improvement in the production of extracellular lignin peroxidases by Phanerochaete chrysosporium: effect of solid manganese(IV)oxide , 1989, Applied Microbiology and Biotechnology.

[3]  M. Leisola,et al.  Factors affecting lignin degradation in lignocellulose by Phanerochaete chrysosporium , 1984, Archives of Microbiology.

[4]  Lew Christov,et al.  Waste Water Bioremediation in the Pulp and Paper Industry , 2003 .

[5]  V. Singhal,et al.  Phytoremediation of pulp and paper mill and distillery effluents by channel grass (Vallisneria spiralis). , 2003 .

[6]  M. Cho,et al.  Laccase production from repeated batch cultures using free mycelia of Trametes sp. , 2002 .

[7]  S. Helmy,et al.  Biopulping and biobleaching by white rot fungi , 2002 .

[8]  Ángel T. Martínez,et al.  Induction, Isolation, and Characterization of Two Laccases from the White Rot Basidiomycete Coriolopsis rigida , 2002, Applied and Environmental Microbiology.

[9]  M. Gold,et al.  Lignin peroxidase oxidation of veratryl alcohol: effects of the mutants H82A, Q222A, W171A, and F267L. , 2002, Biochemistry.

[10]  R. Blanchette,et al.  Bacterial Biodegradation of Extractives and Patterns of Bordered Pit Membrane Attack in Pine Wood , 2000, Applied and Environmental Microbiology.

[11]  L. Nain,et al.  Solid state fermentation of sorghum straw with cellulolytic Trichoderma viride strains and its effect on wheat in conjunction with Azotobacter chroococcum strain W5. , 2000 .

[12]  F. J. Ruiz-Dueñas,et al.  Description of a Versatile Peroxidase Involved in the Natural Degradation of Lignin That Has Both Manganese Peroxidase and Lignin Peroxidase Substrate Interaction Sites* , 1999, The Journal of Biological Chemistry.

[13]  I. Reid.  Biological pulping in paper manufacture , 1991 .

[14]  M. Tien,et al.  Selection and characterization of mutants of Phanerochaete chrysosporium exhibiting ligninolytic activity under nutrient-rich conditions , 1990, Applied and environmental microbiology.

[15]  P. Trotter.  Biotechnology in the pulp and paper industry: a review. Part 2: upgrading pulp properties, papermaking, effluent treatment, and converting biomass to fuels and chemicals. , 1990 .

[16]  R. Singh,et al.  Biodegradation of lignocellulosics in solid state fermentation (SSF) by Pleurotus Spp , 1989 .

[17]  K. Eriksson,et al.  Microbial degradation of chlorolignins. , 1985, Environmental science & technology.

[18]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[19]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.