Multiparametric analysis of cellular and subcellular function using spectral imaging

Development of fluorescent probes which sense cellular constituents (metabolites, ions) has revolutionized studies of cell physiology. To take full advantage of these probes for physiological studies, techniques which simultaneously monitor signal from multiple probes with high temporal resolution are required. In addition,the response of unique cells within a population may be heterogeneous making spatial sampling important for specific applications.

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