Multiplexed Data Independent Acquisition for Comparative Proteomics

Each scan isolates and fragments five 4 m/z – wide precursor ranges randomly chosen from a predefined list of consecutive, adjacent ranges. In this example, the mass range from 500-900 m/z is sampled every 20 scan events. Step 1: Generate a list of isolation windows to be sampled (in this example, 100 4 m/z wide windows 500-900 m/z) Step 2: Build a scan by removing 5 isolation windows at random from the isolation window list, repeat until the isolation window list is empty Step 3: Repopulate the isolation window list, go to step 2. Q-Exactive Isolation Window Uniformity