2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain

Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol‐producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI‐TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.

[1]  B. Svensson,et al.  Secretomics identifies Fusarium graminearum proteins involved in the interaction with barley and wheat. , 2012, Molecular plant pathology.

[2]  V. De Re,et al.  2-D gel electrophoresis: constructing 2D-gel proteome reference maps. , 2012, Methods in molecular biology.

[3]  L. Hoffmann,et al.  Comparative Analysis of Genetic Chemotyping Methods for Fusarium: Tri13 Polymorphism Does not Discriminate between 3‐ and 15‐acetylated Deoxynivalenol Chemotypes in Fusarium graminearum , 2011 .

[4]  S. Walkowiak,et al.  Tri6 Is a Global Transcription Regulator in the Phytopathogen Fusarium graminearum , 2011, PLoS pathogens.

[5]  H. Giese,et al.  Two Novel Classes of Enzymes Are Required for the Biosynthesis of Aurofusarin in Fusarium graminearum* , 2011, The Journal of Biological Chemistry.

[6]  Gerhard Adam,et al.  FGDB: revisiting the genome annotation of the plant pathogen Fusarium graminearum , 2010, Nucleic Acids Res..

[7]  K. O’Donnell,et al.  Nivalenol-type populations of Fusarium graminearum and F. asiaticum are prevalent on wheat in southern Louisiana. , 2011, Phytopathology.

[8]  Xing-Ming Zhao,et al.  FGsub: Fusarium graminearum protein subcellular localizations predicted from primary structures , 2010, BMC Systems Biology.

[9]  C. Fanelli,et al.  Natural functions of mycotoxins and control of their biosynthesis in fungi , 2010, Applied Microbiology and Biotechnology.

[10]  T. Ward,et al.  A comparison of aggressiveness and deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat , 2010, European Journal of Plant Pathology.

[11]  L. Hoffmann,et al.  Fusarium head blight and associated mycotoxin occurrence on winter wheat in Luxembourg in 2007/2008 , 2010, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[12]  L. Hoffmann,et al.  Genetic Fusarium chemotyping as a useful tool for predicting nivalenol contamination in winter wheat. , 2010, International journal of food microbiology.

[13]  N. H. Spliid,et al.  Investigation of the effect of nitrogen on severity of Fusarium head blight in barley. , 2010, Journal of proteomics.

[14]  C. Rampitsch,et al.  The phosphoproteome of Fusarium graminearum at the onset of nitrogen starvation , 2010, Proteomics.

[15]  R. Apweiler,et al.  Finding one's way in proteomics: a protein species nomenclature , 2009, Chemistry Central journal.

[16]  Sang-Yun Cho,et al.  Proteomic analysis of fungal host factors differentially expressed by Fusarium graminearum infected with Fusarium graminearum virus-DK21. , 2009, Virus research.

[17]  J. Manners,et al.  Nutrient profiling reveals potent inducers of trichothecene biosynthesis in Fusarium graminearum. , 2009, Fungal genetics and biology : FG & B.

[18]  N. Ponts,et al.  Fusarium response to oxidative stress by H2O2 is trichothecene chemotype-dependent. , 2009, FEMS microbiology letters.

[19]  Xiaoying Zhou,et al.  Global gene regulation by Fusarium transcription factors Tri6 and Tri10 reveals adaptations for toxin biosynthesis , 2009, Molecular microbiology.

[20]  T. Kinzy,et al.  Coordination of Eukaryotic Translation Elongation Factor 1A (eEF1A) Function in Actin Organization and Translation Elongation by the Guanine Nucleotide Exchange Factor eEF1Bα* , 2009, Journal of Biological Chemistry.

[21]  Rolf Apweiler,et al.  The speciation of the proteome , 2008, Chemistry Central journal.

[22]  L. Harris,et al.  Proteomic analyses of Fusarium graminearum grown under mycotoxin‐inducing conditions , 2008, Proteomics.

[23]  D. Geiser,et al.  An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America. , 2008, Fungal genetics and biology : FG & B.

[24]  J. Renaut,et al.  Quantitative proteomic analysis of short photoperiod and low-temperature responses in bark tissues of peach (Prunus persica L. Batsch) , 2008, Tree Genetics & Genomes.

[25]  Christina A. Cuomo,et al.  Comparative proteomics of extracellular proteins in vitro and in planta from the pathogenic fungus Fusarium graminearum , 2007, Proteomics.

[26]  A. Laroche,et al.  Identification of differentially regulated proteins in response to a compatible interaction between the pathogen Fusarium graminearum and its host, Triticum aestivum , 2006, Proteomics.

[27]  F. Delalande,et al.  Diversity of the exoproteome of Fusarium graminearum grown on plant cell wall , 2005, Current Genetics.

[28]  Zhengqiang Ma,et al.  Differential proteomic analysis of proteins in wheat spikes induced by Fusarium graminearum , 2005, Proteomics.

[29]  F. Minervini,et al.  Toxicity and apoptosis induced by the mycotoxins nivalenol, deoxynivalenol and fumonisin B1 in a human erythroleukemia cell line. , 2004, Toxicology in vitro : an international journal published in association with BIBRA.

[30]  Kim Nasmyth,et al.  Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements , 1987, Cell.

[31]  M. Zivy,et al.  Technical improvements in two‐dimensional electrophoresis increase the level of genetic variation detected in wheat‐seedling proteins , 1986 .

[32]  H. Kurata,et al.  Chemotaxonomy of Gibberella zeae with special reference to production of trichothecenes and zearalenone , 1983, Applied and environmental microbiology.

[33]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.