Antibacterial activity of a grape seed extract and its fractions against Campylobacter spp.

In this study, the antibacterial activity of a grape seed extract (GSE) was examined against different Campylobacter strains. Growth inhibition was in the range from 5.08 to 6.97 log CFU/ml, demonstrating the strong capacity of the GSE to inhibit Campylobacter growth. Further dilution of the extract showed a minimal inhibitory concentration (MIC) of 20 mg/l and a minimal bactericidal concentration (MBC) of 60 mg/l against Campylobacter jejuni. GSE was fractioned by RP-HPLC and phenolic composition was determined by HPLC-DAD and HPLC-MS. The phenolic profile of GSE mainly consisted on flavonols, phenolic acids, catechins and proanthocyanidins, and anthocyanins. Among them, catechins and proanthocyanidins were the major compounds, representing 77.6% of total phenolic compounds determined. The analysis of the antibacterial activity against C. jejuni of the collected fractions showed that phenolic acids, catechins and proanthocyanidins were the main responsible of the behavior observed. These results showed that identification and quantification of the individual phenolic compounds of GSE could be feasible to standardize the production process to obtain an enriched extract potentially useful to control Campylobacter in the food chain.

[1]  G. Nychas,et al.  Antilisterial activities of polyphenol-rich extracts of grapes and vinification byproducts. , 2009, Journal of agricultural and food chemistry.

[2]  M. Yılmaz,et al.  Effect of grape pomace extracts obtained from different grape varieties on microbial quality of beef patty. , 2011, Journal of food science.

[3]  K. Shetty,et al.  Inhibitory potential of tea polyphenolics and influence of extraction time against Helicobacter pylori and lack of inhibition of beneficial lactic acid bacteria. , 2011, Journal of medicinal food.

[4]  F. Saura-calixto,et al.  Effect of grape seed extract on growth performance, protein and polyphenol digestibilities, and antioxidant activity in chickens , 2010 .

[5]  C. Badet,et al.  In vitro study of antioxidant capacity and antibacterial activity on oral anaerobes of a grape seed extract. , 2009 .

[6]  M. S. Brewer,et al.  Effect of grape seed extract on oxidative, color and sensory stability of a pre-cooked, frozen, re-heated beef sausage model system. , 2011, Meat science.

[7]  M. Blaser,et al.  Clinical aspects of Campylobacter jejuni and Campylobacter coli infections. , 2008 .

[8]  A. Mustapha,et al.  Antimicrobial and antioxidant activities of natural extracts in vitro and in ground beef. , 2004, Journal of food protection.

[9]  R. Mandrell,et al.  Antimicrobial activities of tea catechins and theaflavins and tea extracts against Bacillus cereus. , 2006, Journal of food protection.

[10]  J. M. Torres-Rodríguez,et al.  In Vitro Susceptibility of Sporothrix schenckii to Six Antifungal Agents Determined Using Three Different Methods , 2007, Antimicrobial Agents and Chemotherapy.

[11]  Victor R. Preedy,et al.  Nuts and Seeds in Health and Disease Prevention , 2011 .

[12]  J. Silvan,et al.  Alternative strategies to use antibiotics or chemical products for controlling Campylobacter in the food chain , 2012 .

[13]  M. M. Cowan.  Plant Products as Antimicrobial Agents , 1999, Clinical Microbiology Reviews.

[14]  C. Sánchez-Moreno,et al.  Bioconversion of anthocyanin glycosides by Bifidobacteria and Lactobacillus , 2009 .

[15]  K. Shetty,et al.  Cranberry synergies for dietary management of Helicobacter pylori infections , 2005 .

[16]  R. Peces,et al.  Phenolic compounds in skins and seeds of ten grape Vitis vinifera varieties grown in a warm climate , 2006 .

[17]  Jie Zhou,et al.  Assessment of the antibacterial activity and the antidiarrheal function of flavonoids from bayberry fruit. , 2011, Journal of agricultural and food chemistry.

[18]  P. L. Rhodes,et al.  Antilisterial activity of grape juice and grape extracts derived from Vitis vinifera variety Ribier. , 2006, International journal of food microbiology.

[19]  A. Lastovica.  Emerging Campylobacter spp.: the Tip of the Iceberg , 2006 .

[20]  P. Cheung,et al.  Spectrophotometric determination of phenolic compounds by enzymatic and chemical methods--a comparison of structure--activity relationship. , 2007, Journal of agricultural and food chemistry.

[21]  C. Braicu,et al.  Antibacterial action of an aqueous grape seed polyphenolic extract , 2011 .

[22]  B. Bartolomé,et al.  Antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria. , 2010, Research in microbiology.

[23]  Xiuping Jiang,et al.  Antibacterial Effects of Grape Extracts on Helicobacter pylori , 2008, Applied and Environmental Microbiology.

[24]  Margie D. Lee,et al.  Campylobacter in Poultry: Filling an Ecological Niche , 2006, Avian diseases.

[25]  A. Lamb,et al.  Recent advances in understanding the antibacterial properties of flavonoids. , 2011, International journal of antimicrobial agents.

[26]  K. Jones,et al.  Cattle and sheep farms as reservoirs of Campylobacter , 2003, Journal of applied microbiology.

[27]  Emin Burçin Özvural,et al.  Grape seed flour is a viable ingredient to improve the nutritional profile and reduce lipid oxidation of frankfurters. , 2011, Meat science.

[28]  J. Canadanovic-Brunet,et al.  Polyphenolic Composition and Antioxidant Activities of Grape Seed Extract , 2008 .

[29]  M. G. Johnson,et al.  Effect of organic acids and plant extracts on Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium in broth culture model and chicken meat systems. , 2009, Journal of food science.

[30]  M. Recio,et al.  Medicinal plants and antimicrobial activity. , 2005, Journal of ethnopharmacology.

[31]  J. Collins,et al.  Prevalence of thermophilic Campylobacter species in cats and dogs in two animal shelters in Ireland , 2006, Veterinary Record.

[32]  H. Alakomi,et al.  Berry Phenolics: Antimicrobial Properties and Mechanisms of Action Against Severe Human Pathogens , 2006, Nutrition and cancer.

[33]  D. Yiğit,et al.  Antioxidant and antimicrobial activities of methanol and water extracts of fruits, leaves and seeds of Vitis vinifera L. cv. Karaerik. , 2009 .

[34]  A. Mustapha,et al.  Effects of plant extracts on microbial growth, color change, and lipid oxidation in cooked beef. , 2007, Food microbiology.

[35]  A. Carrascosa,et al.  Antimicrobial activity of phenolic compounds of wine against Campylobacter jejuni , 2009 .

[36]  T. M. Vieira,et al.  Wine industry residue as antioxidant in cooked chicken meat , 2010 .

[37]  K. K. Sakariah,et al.  Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts , 2003 .

[38]  M. Lila,et al.  Effects of Food Processing on Blueberry Antiproliferation and Antioxidant Activity , 2006 .

[39]  Efsa Publication,et al.  The Community Summary Report on Trends and Sources of Zoonoses in 2008 , 2010 .

[40]  N. Montes-Villanueva,et al.  Wine industry residues extracts as natural antioxidants in raw and cooked chicken meat during frozen storage. , 2011, Meat science.

[41]  S. A. Rasool,et al.  Antibacterial activity directed isolation of compounds from Punica granatum. , 2007, Journal of food science.

[42]  Efsa Journal,et al.  European Food Safety Authority, European Centre for Disease Prevention and Control; The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2009 , 2011 .

[43]  Simon F. Park.  The physiology of Campylobacter species and its relevance to their role as foodborne pathogens. , 2002, International journal of food microbiology.

[44]  I. López-Expósito,et al.  Identification of antibacterial peptides from ovine αs2-casein , 2006 .

[45]  Osman Sagdic,et al.  Determination of antibacterial effects and total phenolic contents of grape (Vitis vinifera L.) seed extracts , 2006 .

[46]  S. O'Brien,et al.  Campylobacters as zoonotic pathogens: a food production perspective. , 2007, International journal of food microbiology.

[47]  M. Blaser,et al.  Mechanisms of Antibiotic Resistance in Campylobacter , 2008 .