Combined therapy using low level laser and chitosan-policaju hydrogel for wound healing.

We have evaluated the effect of POLI-CHI hydrogel based on policaju (POLI) from cashew tree (Anacardium occidentale L.) gum and chitosan (CHI), associated or not with Low level laser therapy (LLLT), in wound healing. Sixty male Wistar rats were assigned into four groups: POLI-CHI hydrogel (H); LLLT (L); POLI-CHI with LLLT (HL) and saline control (C). Macroscopic evaluations were carried out using clinical observations and area measurements, as well as microscopic analysis by histological criteria. H and HL presented more esthetical scar tissue and larger wound contraction compared to C. Histopathological analyzes showed: stronger presence of fibrin-leukocyte crust in L and HL at day 3; stronger collagen presence in H, L and HL; weak presence of focal necrosis at 7 and 14days in H; weak neutrophilic exudate in H, L and HL; regression of the vascular neoformation at 7days in H, and modulation of the same in L and HL. These results demonstrated that POLI-CHI contributed to more efficient healing process and modulation of the inflammation; furthermore, the combined use with LLLT subtle potentiated this process.

[1]  John G. Lyons,et al.  Characterisation and controlled drug release from novel drug-loaded hydrogels. , 2008, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[2]  S. Sandhu,et al.  Practices in Wound Healing Studies of Plants , 2011, Evidence-based complementary and alternative medicine : eCAM.

[3]  A. Vicente,et al.  Development and characterization of hydrogels based on natural polysaccharides: policaju and chitosan. , 2014, Materials science & engineering. C, Materials for biological applications.

[4]  M. L. Ferreira,et al.  Effects of low-level laser therapy on wound healing. , 2014, Revista do Colegio Brasileiro de Cirurgioes.

[5]  Daniela Vecchio,et al.  Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. , 2013, Seminars in cutaneous medicine and surgery.

[6]  K Madhumathi,et al.  Wet chemical synthesis of chitosan hydrogel-hydroxyapatite composite membranes for tissue engineering applications. , 2009, International journal of biological macromolecules.

[7]  M. Barzegar,et al.  TREATMENT OF A PIGMENTED HYPERTROPHIC SCAR BY LOW-LEVEL LASER THERAPY (LLLT): A CASE REPORT , 2010 .

[8]  J. N. dos Santos,et al.  Phototherapy improves healing of cutaneous wounds in nourished and undernourished Wistar rats. , 2004, Brazilian dental journal.

[9]  D. Kohane,et al.  HYDROGELS IN DRUG DELIVERY: PROGRESS AND CHALLENGES , 2008 .

[10]  L. Franzén,et al.  A comparative study of three occlusive dressings in the treatment of full-thickness wounds in pigs. , 1997, Journal of the American Academy of Dermatology.

[11]  R. Barbucci,et al.  Polysaccharide-Based Hydrogels: The Key Role of Water in Affecting Mechanical Properties , 2012 .

[12]  Lingli Li,et al.  In vivo evaluation of curcumin nanoformulation loaded methoxy poly(ethylene glycol)-graft-chitosan composite film for wound healing application , 2012 .

[13]  M. A. D. de Oliveira,et al.  Analgesic action of laser therapy (LLLT) in an animal model. , 2008, Medicina oral, patologia oral y cirugia bucal.

[14]  F. Acartürk,et al.  Investigation of the effects of local glutathione and chitosan administration on incisional oral mucosal wound healing in rabbits. , 2013, Colloids and surfaces. B, Biointerfaces.

[15]  Tianhong Dai,et al.  Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects , 2011, Expert review of anti-infective therapy.

[16]  J. Bjordal,et al.  Low-level laser therapy (LLLT) attenuates RhoA mRNA expression in the rat bronchi smooth muscle exposed to tumor necrosis factor-α , 2010, Lasers in Medical Science.

[17]  Giuliana Viegas Schirato,et al.  O polissacarídeo do Anacardium occidentale L. na fase inflamatória do processo cicatricial de lesões cutâneas , 2006 .

[18]  J. C. Cardoso,et al.  Improvement of dermal burn healing by combining sodium alginate/chitosan-based films and low level laser therapy. , 2011, Journal of photochemistry and photobiology. B, Biology.

[19]  A. Singer,et al.  Cutaneous wound healing. , 1999, The New England journal of medicine.

[20]  A. Vicente,et al.  Polysaccharide from Anacardium occidentale L. tree gum (Policaju) as a coating for Tommy Atkins mangoes , 2010 .

[21]  A. Porto,et al.  Immobilization of trypsin on polysaccharide film from Anacardium occidentale L. and its application as cutaneous dressing , 2007 .

[22]  C. Pinfildi,et al.  Low-level laser therapy on the viability of skin flap in rats subjected to deleterious effect of nicotine. , 2011, Photomedicine and laser surgery.

[23]  E. Cevher,et al.  Preparation of fucoidan-chitosan hydrogel and its application as burn healing accelerator on rabbits. , 2008, Biological & pharmaceutical bulletin.

[24]  Martin D. Brennan,et al.  Microfluidic wound bandage: Localized oxygen modulation of collagen maturation , 2013, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[25]  G. Prestwich,et al.  Novel Sulfated Polysaccharides Disrupt Cathelicidins, Inhibit RAGE and Reduce Cutaneous Inflammation in a Mouse Model of Rosacea , 2011, PloS one.

[26]  Kyung-Won Min,et al.  Hemostatic and Wound Healing Properties of Chromolaena odorata Leaf Extract , 2013, ISRN dermatology.