Single-molecule DNA detection using a novel SP1 protein nanopore.

SP1 protein as a new type of biological nanopore is described and is utilized to distinguish single-stranded DNA at the single-molecule level. Using the SP1 nanopore to investigate single molecule detection broadens the existing research areas of pore-forming biomaterials from unsymmetrical biological nanopores to symmetrical biological nanopores. This novel nanopore could provide a good candidate for single-molecule detection and characterization of biomaterial applications.

[1]  G. Fasman,et al.  Single-stranded oligomers and polymers of cytidylic and 2'-deoxycytidylic acids: comparative optical rotatory studies. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[2]  J. M. Scholtz,et al.  Interactions of peptides with a protein pore. , 2005, Biophysical journal.

[3]  Yang Li,et al.  Enhanced translocation of poly(dt)45 through an α-hemolysin nanopore by binding with antibody. , 2011, Chemical communications.

[4]  David Stoddart,et al.  Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore , 2009, Proceedings of the National Academy of Sciences.

[5]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[6]  Yang Li,et al.  Nanopore analysis of β-amyloid peptide aggregation transition induced by small molecules. , 2011, Analytical chemistry.

[7]  Ashraf Aly Hassan,et al.  Selective Oxidation Using Flame Aerosol Synthesized Iron and Vanadium-Doped Nano-TiO2 , 2011 .

[8]  Oded Shoseyov,et al.  The Structural Basis of the Thermostability of SP1, a Novel Plant (Populus tremula) Boiling Stable Protein* , 2004, Journal of Biological Chemistry.

[9]  A. Meller,et al.  Nanopore unzipping of individual DNA hairpin molecules. , 2004, Biophysical journal.

[10]  A. Koster,et al.  SP1 protein-based nanostructures and arrays. , 2008, Nano letters.

[11]  S. Henrickson,et al.  Probing single nanometer-scale pores with polymeric molecular rulers. , 2010, The Journal of chemical physics.

[12]  J. Gouaux,et al.  Structure of Staphylococcal α-Hemolysin, a Heptameric Transmembrane Pore , 1996, Science.

[13]  D. Tsernoglou,et al.  Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states , 1994, Nature.

[14]  M. Troll,et al.  Ionic Current Blockades from DNA and RNA Molecules in the α-Hemolysin Nanopore , 2007 .

[15]  M. Muthukumar,et al.  Translocation of a confined polymer through a hole. , 2001, Physical review letters.

[16]  Artur Makarewicz,et al.  Electronic Supplementary Material ( ESI ) for ChemComm , 2015 .

[17]  M. Niederweis,et al.  Single-molecule DNA detection with an engineered MspA protein nanopore , 2008, Proceedings of the National Academy of Sciences.

[18]  A. Altman,et al.  Characterization of SP1, a Stress-Responsive, Boiling-Soluble, Homo-Oligomeric Protein from Aspen1 , 2002, Plant Physiology.

[19]  D. Branton,et al.  Rapid nanopore discrimination between single polynucleotide molecules. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[20]  D. Branton,et al.  Characterization of individual polynucleotide molecules using a membrane channel. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[21]  L. Gu,et al.  Method of creating a nanopore-terminated probe for single-molecule enantiomer discrimination. , 2009, Analytical chemistry.