Novel carboxylation treatment and characterization of multiwalled carbon nanotubes for simultaneous sensitive determination of adenine and guanine in DNA

A method is presented for the carboxylation of multiwalled carbon nanotubes (MWCNTs) via a two-step process. The hydroxy groups of MWCNTs were first reacted with epichlorohydrin, then with iminodiacetic acid. The resulting MWCNTs were characterized by means of Fourier transform infrared spectroscopy and transmission electron microscopy. The glassy carbon electrode modified with the MWCNTs thus prepared exhibited enhanced electrocatalytic activity and good stability for the determination of guanine and adenine in pH 7.0 phosphate buffer solution. The experimental parameters were optimized, and a direct electrochemical method was developed for the simultaneous determination of guanine and adenine. The detection limits (at S/N = 3) for guanine and adenine are 0.02 and 0.08 μM, respectively. A sensitive method was also developed for the determination of guanine and adenine in calf thymus DNA.

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