Influence of silica gel in production of diacylglycerol via enzymatic glycerolysis of palm olein.

Enzymatic glycerolysis was explored in this paper for the production of diacylglycerol (DAG) oils from palm olein. Three commercial enzymes, Lipozyme TL IM, Lipozyme RM IM and Novozym 435 were used for their ability to synthesize DAG in a solvent-free system. Novozym 435 was found to be the more effective enzyme, resulting in a high DAG production even in the absence of an adsorbent such as silica gel. The yields of DAG were between 43 and 50 wt-%. Lipozyme TL IM and RM IM, being supported on hydrophilic materials, require an adsorbent to allow slow release of glycerol for reaction with the enzyme and oil. In the absence of silica, no reaction was observed. The success of the reaction is therefore very dependent on the amount of silica used. The yields of DAG using Lipozyme TL IM and RM IM were 52 and 45 wt-%, respectively. In addition, the degree of reduction in tocopherols and tocotrienols appeared correlated with the efficacy of the glycerolysis reaction. Changes in the slip melting points and solid fat contents of the products are indicative of the reaction occurring.

[1]  A. H-Kittikun,et al.  Continuous production of monoacylglycerols from palm olein in packed-bed reactor with immobilized lipase PS , 2008 .

[2]  P. Dutta,et al.  Stability of Minor Lipid Components with Emphasis on Phytosterols During Chemical Interesterification of a Blend of Refined Olive Oil and Palm Stearin , 2008 .

[3]  A. H-Kittikun,et al.  Kinetic study of glycerolysis of palm olein for monoacylglycerol production by immobilized lipase , 2007 .

[4]  C. Tan,et al.  Production of a diacylglycerol-enriched palm olein using lipase-catalyzed partial hydrolysis: Optimization using response surface methodology , 2007 .

[5]  H. Sovová,et al.  Kinetic modeling of glycerolysis–hydrolysis of canola oil in supercritical carbon dioxide media using equilibrium data , 2006 .

[6]  A. Chambers,et al.  Effect of tocotrienols on the growth of a human breast cancer cell line in culture , 1995, Lipids.

[7]  Xuebing Xu,et al.  Process optimization using response surface design and pilot plant production of dietary diacylglycerols by lipase-catalyzed glycerolysis. , 2005, Journal of agricultural and food chemistry.

[8]  Xuebing Xu,et al.  Diacylglycerol synthesis by enzymatic glycerolysis: Screening of commercially available lipases , 2005 .

[9]  Xuebing Xu,et al.  Enzymatic production of monoacylglycerols containing polyunsaturated fatty acids through an efficient glycerolysis system. , 2005, Journal of agricultural and food chemistry.

[10]  Kazuhiro Nakanishi,et al.  Diacylglycerol production in a packed bed bioreactor , 2005 .

[11]  A. H-Kittikun,et al.  Glycerolysis of palm olein by immobilized lipase PS in organic solvents , 2004 .

[12]  B. S. Baharin,et al.  Lipase‐catalysed production and chemical composition of diacylglycerols from soybean oil deodoriser distillate , 2004 .

[13]  C. Torres,et al.  Lipase-catalyzed glycerolysis of an oil rich in eicosapentaenoic acid residues , 2002, Biotechnology Letters.

[14]  K. Maki,et al.  Consumption of diacylglycerol oil as part of a reduced-energy diet enhances loss of body weight and fat in comparison with consumption of a triacylglycerol control oil. , 2002, The American journal of clinical nutrition.

[15]  T. Yamane,et al.  Enzymatic synthesis of symmetrical 1,3-diacylglycerols by direct esterification of glycerol in solvent-free system , 1999 .

[16]  J. Aracil,et al.  Reactions of olive oil and glycerol over immobilized lipases , 1998 .

[17]  B. Mattiasson,et al.  Preparation of diglycerides by lipase-catalyzed alcoholysis of triglycerides , 1997 .

[18]  D. Combes,et al.  The role of silica gel in lipase-catalyzed esterification reactions of high-polar substrates , 1997 .

[19]  M. Schneider,et al.  Enzymatic esterification of glycerol III. Lipase-catalyzed synthesis of regioisomerically pure 1,3-sn-diacylglycerols and 1 (3)-rac-monoacylglycerols derived from unsaturated fatty acids , 1996 .

[20]  F. Plou,et al.  High-yield production of mono- and di-oleylglycerol by lipase-catalyzed hydrolysis of triolein , 1996 .

[21]  H. Ghazali,et al.  Enzymatic transesterification of palm olein with nonspecific and 1,3-specific lipases , 1995 .

[22]  Y. Man,et al.  Composition of crystals of palm olein formed at room temperature , 1995 .

[23]  K. Parkin,et al.  Continuous reactor for enzymic glycerolysis of butteroil in the absence of solvent , 1995 .

[24]  T. Yamane,et al.  High-yield diacylglycerol formation by solid-phase enzymatic glycerolysis of hydrogenated beef tallow , 1994 .

[25]  M. Schneider,et al.  Enzymatic esterification of glycerol I. Lipase-catalyzed synthesis of regioisomerically pure 1,3-sn-diacylglycerols , 1992 .

[26]  L. Packer,et al.  Palm oil vitamin E protects against ischemia/reperfusion injury in the isolated perfused Langendorff heart , 1992 .