Contact-dependent transfer of a lysosomal enzyme from lymphocytes to fibroblasts.

In this study we have examined the mechanism underlying the contact-mediated transfer of a lysosomal enzyme from lymphocytes to fibroblasts in culture. We found that although antibody against the mannose 6-phosphate lysosomal targetting receptor (MPR) completely inhibited fibroblast uptake of the lysosomal enzyme beta-glucuronidase (Gus) from the culture medium, it had no effect on the transfer of the enzyme from normal lymphocytes. In contrast, the presence of antibody that prevented the adhesion of the lymphocytes to the fibroblasts inhibited Gus acquisition but had no effect on endocytosis. Immunogold electron microscopy of the contact site between the two types of cell showed that the transfer of Gus involved uncoated vesicles localized near the cell surface of the fibroblast at sites of contact with the lymphocytes. The acquired lymphocyte enzyme was shown to be transported to the fibroblast lysosomes.

[1]  J. Rodman,et al.  Contact formation and transfer of mannose BSA gold from macrophages to cocultured fibroblasts. , 1991, Experimental cell research.

[2]  George Bou-Gharios,et al.  The activation of resting lymphocytes is accompanied by the biogenesis of lysosomal organelles , 1990, European journal of immunology.

[3]  P. Bongrand,et al.  Use of conjugates made between a cytolytic T cell clone and target cells to study the redistribution of membrane molecules in cell contact areas. , 1990, Journal of cell science.

[4]  George Bou-Gharios,et al.  Adhesion of lymphoid cells to fibroblasts in tissue culture. , 1989, Cellular immunology.

[5]  J. C. Martin,et al.  Intracellular localization of beta-glucuronidase in fibroblasts after direct transfer from macrophages. , 1988, The Biochemical journal.

[6]  J. Moss,et al.  A simple technique for in situ embedding of monolayer cultures in Lowicryl K4M , 1988, Journal of microscopy.

[7]  A. Engel,et al.  Monoclonal antibody analysis of mononuclear cells in myopathies. V: Identification and quantitation of T8+ cytotoxic and T8+ suppressor cells , 1988, Annals of neurology.

[8]  George Bou-Gharios,et al.  Cell contact induces the synthesis of a lysosomal enzyme precursor in lymphocytes and its direct transfer to fibroblasts. , 1988, Biochimica et biophysica acta.

[9]  S. Kornfeld.  Trafficking of lysosomal enzymes 1 , 1987, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[10]  L. Orci,et al.  Ligands internalized through coated or noncoated invaginations follow a common intracellular pathway. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[11]  I. Young.  Bone Marrow Transplantation for Treatment of Lysosomal Storage Diseases , 1987 .

[12]  H. Galjaard,et al.  Immunocytochemical localization of lysosomal acid phosphatase in normal and "I-cell" fibroblasts. , 1987, European journal of cell biology.

[13]  K. Hugh-Jones.  BONE MARROW TRANSPLANTATION FOR THE TREATMENT OF LYSOSOMAL STORAGE DISEASES , 1986, Pediatric Research.

[14]  T. Partridge,et al.  Role of cell adhesion in contact-dependent transfer of a lysosomal enzyme from lymphocytes to fibroblasts. , 1986, Journal of cell science.

[15]  A. Schwartz,et al.  Receptor-mediated endocytosis. , 1986, The Journal of clinical investigation.

[16]  M. Dean,et al.  Fibroblasts acquire beta-glucuronidase by direct and indirect transfer during co-culture with macrophages. , 1985, Experimental cell research.

[17]  B. Winchester,et al.  Direct enzyme transfer from lymphocytes corrects a lysosomal storage disease. , 1985, Biochemical and biophysical research communications.

[18]  I. Mellman.  Membrane recycling during endocytosis , 1985 .

[19]  D. Watt,et al.  Direct enzyme transfer from lymphocytes is specific , 1983, Nature.

[20]  J. Yang,et al.  Arylsulfatase in natural killer cells: its possible role in cytotoxicity. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Willingham.  An alternative fixation-processing method for preembedding ultrastructural immunocytochemistry of cytoplasmic antigens: the GBS (glutaraldehyde-borohydride-saponin) procedure. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[22]  W. Sly,et al.  The role of intermediate vesicles in the adsorptive endocytosis and transport of ligand to lysosomes by human fibroblasts , 1983, The Journal of cell biology.

[23]  M. Dean,et al.  Identification of rabbit and mouse beta-glucuronidases in human fibroblasts following direct interaction with lymphocytes. , 1982, Biochimica et biophysica acta.

[24]  M. Dean,et al.  Acquisition of beta-glucuronidase activity by deficient fibroblasts during direct contact with lymphoid cells. , 1982, Journal of cell science.

[25]  L. Orci,et al.  Non-coated membrane invaginations are involved in binding and internalization of cholera and tetanus toxins , 1982, Nature.

[26]  I. Weissman,et al.  Differences in the migration of B and T lymphocytes: organ-selective localization in vivo and the role of lymphocyte-endothelial cell recognition. , 1982, Journal of immunology.

[27]  S. Shimizu,et al.  Rapid isolation and lipid characterization of plasma membranes from normal and malignant lymphoid cells of mouse. , 1981, Biochimica et biophysica acta.

[28]  I. Pastan,et al.  Morphologic study of the internalization of a lysosomal enzyme by the mannose 6-phosphate receptor in cultured Chinese hamster ovary cells. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[29]  M. Dean,et al.  Direct transfer of a lysosomal enzyme from lymphoid cells to deficient fibroblasts , 1981, Nature.

[30]  K. von Figura,et al.  Transport and processing of lysosomal enzymes by smooth muscle cells and endothelial cells. , 1981, Experimental cell research.

[31]  S. Singer,et al.  The antibody-induced clustering and endocytosis of HLA antigens on cultured human fibroblasts , 1980, Cell.

[32]  E. Butcher,et al.  Evidence of continuous evolutionary change in structures mediating adherence of lymphocytes to specialised venules , 1979, Nature.

[33]  A. Siebert.  Septate-like junctions between cells of the immune system in vivo. , 1979, Cell biology international reports.

[34]  L. Rome,et al.  Two species of lysosomal organelles in cultured human fibroblasts , 1979, Cell.

[35]  G. Gregoriadis,et al.  Enzyme therapy in genetic diseases , 1979, Nature.

[36]  K. von Figura,et al.  An alternative hypothesis of cellular transport of lysosomal enzymes in fibroblasts. Effect of inhibitors of lysosomal enzyme endocytosis on intra- and extra-cellular lysosomal enzyme activities. , 1978, The Biochemical journal.

[37]  K. Holbrook,et al.  Contact sites between lymphoid cells of the bursa of fabricius, in vivo and in vitro , 1977, The Anatomical record.

[38]  H. Kawanishi.  Morphologic association of lymphocytes with hepatocytes in chronic liver disease. , 1977, Archives of pathology & laboratory medicine.

[39]  J. Kaplan.  Cell contact induces an increase in pinocytotic rate in cultured epithelial cells , 1976, Nature.

[40]  E. Reith,et al.  The ultrastructure of mouse lymph node venules and the passage of lymphocytes across their walls. , 1974, Journal of ultrastructure research.

[41]  D. Nicol,et al.  Uptake of beta-glucuronidase by deficient human fibroblasts. , 1973, Laboratory investigation; a journal of technical methods and pathology.

[42]  K. Paigen,et al.  Biochemical and genetic evidence for a macromolecular -glucuronidase complex in microsomal membranes. , 1973, Journal of molecular biology.

[43]  M. Ziff,et al.  Electron microscopic studies of lymphoid cells in the rheumatoid synovial membrane. , 1973, Arthritis and rheumatism.

[44]  B. Morris,et al.  The traffic of cells through tissues: a study of peripheral lymph in sheep. , 1970, Journal of anatomy.

[45]  W. Fishman,et al.  A POSTCOUPLING TECHNIQUE FOR β-GLUCURONIDASE EMPLOYING THE SUBSTRATE, NAPHTHOL AS-BI-β-d-GLUCOSIDURONIC ACID , 1965 .

[46]  R. Moore,et al.  CYTOPLASMIC INTERACTION BETWEEN MACROPHAGES AND LYMPHOCYTIC CELLS IN ANTIBODY SYNTHESIS. , 1964, Science.

[47]  S. Malhotra.  Fixation for Electron Microscopy , 1963, Nature.

[48]  G. Palade,et al.  JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA , 1963, The Journal of cell biology.

[49]  A. George.  The metabolic basis of inherited disease , 1961 .

[50]  I. Mellman,et al.  The biogenesis of lysosomes. , 1989, Annual review of cell biology.

[51]  F. N. Ghadially.  Endocytotic structures and cell processes , 1988 .

[52]  K. von Figura,et al.  Lysosomal enzymes and their receptors. , 1986, Annual review of biochemistry.

[53]  S. Singer,et al.  Reorientation of the Golgi apparatus and the microtubule organizing center: is it a means to polarize cell-mediated cytotoxicity? , 1985, Advances in experimental medicine and biology.

[54]  P. Henkart.  Mechanism of lymphocyte-mediated cytotoxicity. , 1985, Annual review of immunology.

[55]  K. Henry,et al.  BONE MARROW TRANSPLANTATION IN MUCOPOLYSACCHARIDOSES , 1984 .

[56]  F. Paronetto.  Immunologic aspects of liver diseases. , 1970, Progress in liver diseases.

[57]  N. Green.  Electron microscopy of the immunoglobulins. , 1969, Advances in immunology.

[58]  P. Siekevitz,et al.  The Ultrastructure of the Animal Cell , 1967 .

[59]  J. Gowans.  Life-span, recirculation, and transformation of lymphocytes. , 1966, International review of experimental pathology.