A novel synthetic tissue-adhesive hydrogel using a crosslinkable polymeric micelle.

We prepared a novel tissue-adhesive hydrogel by using a polymeric micelle consisting of an aldehyde-terminated poly(ethylene glycol)-poly(D,L-lactide) (PEG-PLA) block polymer. A Schiff base is chemically formed between the amino groups in a polyallylamine and the aldehyde groups on the surface of polymeric micelles. The hydrogel was formed in approximately 2 s when the polymeric micelle solution and polyallylamine solution are mixed in vitro. The hydrogel was rapidly formed in vivo, and it adhered to a tissue surface. Our novel tissue-adhesive hydrogel creates no risk of infectious contaminations, because it consists of only synthetic materials. Further, PEG and PLA are known to be biocompatible and noncytotoxic. The results obtained in the present study show that a hydrogel prepared by the formation of a Schiff base between aldehyde and amine groups will potentially address the need for novel tissue-adhesive materials.

[1]  M. Czerny,et al.  Collagen patch coated with fibrin glue components. Treatment of suture hole bleedings in vascular reconstruction. , 2000, The Journal of cardiovascular surgery.

[2]  L. Kavoussi,et al.  Local hemostasis during laparoscopic partial nephrectomy using biodegradable hydrogels: initial porcine results. , 2002, Journal of endourology.

[3]  T. MacGillivray.  Fibrin Sealants and Glues , 2003, Journal of cardiac surgery.

[4]  Y. Nakayama,et al.  Photocurable surgical tissue adhesive glues composed of photoreactive gelatin and poly(ethylene glycol) diacrylate. , 1999, Journal of biomedical materials research.

[5]  G. Cruise,et al.  A tissue sealant based on reactive multifunctional polyethylene glycol. , 2001, Journal of biomedical materials research.

[6]  Akeo Hagiwara,et al.  How safe are the xenogeneic hemostats? — Report of a case of severe systemic allergic reaction , 1995, Surgery Today.

[7]  T. Taguchi,et al.  Bonding of soft tissues using a novel tissue adhesive consisting of a citric acid derivative and collagen , 2004 .

[8]  P. Campbell,et al.  Evaluation of a sprayable polyethylene glycol adhesion barrier in a porcine efficacy model. , 2001, Human reproduction.

[9]  A. Singer,et al.  A review of the literature on octylcyanoacrylate tissue adhesive. , 2004, American journal of surgery.

[10]  A. Turner,et al.  Evaluation of a novel hemostatic device in an ovine parenchymal organ bleeding model of normal and impaired hemostasis. , 2002, Journal of biomedical materials research.

[11]  M. Yokoyama,et al.  Drug targeting with nano-sized carrier systems , 2005, Journal of Artificial Organs.

[12]  Takashi Okada,et al.  The Reactive Polymeric Micelle Based on An Aldehyde-Ended Poly(ethylene glycol)/Poly(lactide) Block Copolymer , 1998 .

[13]  A. Kaye,et al.  Evaluation of the use of BioGlue® in neurosurgical procedures , 2003, Journal of Clinical Neuroscience.

[14]  M. Karck,et al.  The use of gelatin-resorcin-formalin glue in acute aortic dissection type A. , 1999, European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery.

[15]  Y. Ikada,et al.  A new biological glue from gelatin and poly (L-glutamic acid). , 1996, Journal of biomedical materials research.

[16]  K. Kataoka,et al.  A Novel Reactive Polymeric Micelle with Aldehyde Groups on Its Surface , 1995 .

[17]  C. Dohno,et al.  Stable, specific, and reversible base pairing via Schiff base. , 2005, Journal of the American Chemical Society.