A reference map of the human binary protein interactome

Global insights into cellular organization and genome function require comprehensive understanding of the interactome networks that mediate genotype–phenotype relationships 1 , 2 . Here we present a human ‘all-by-all’ reference interactome map of human binary protein interactions, or ‘HuRI’. With approximately 53,000 protein–protein interactions, HuRI has approximately four times as many such interactions as there are high-quality curated interactions from small-scale studies. The integration of HuRI with genome 3 , transcriptome 4 and proteome 5 data enables cellular function to be studied within most physiological or pathological cellular contexts. We demonstrate the utility of HuRI in identifying the specific subcellular roles of protein–protein interactions. Inferred tissue-specific networks reveal general principles for the formation of cellular context-specific functions and elucidate potential molecular mechanisms that might underlie tissue-specific phenotypes of Mendelian diseases. HuRI is a systematic proteome-wide reference that links genomic variation to phenotypic outcomes. A human binary protein interactome map that includes around 53,000 protein–protein interactions involving more than 8,000 proteins provides a reference for the study of human cellular function in health and disease.

Patrick Aloy | Gary D. Bader | Yang Wang | Gary D Bader | Yu Xia | Soon Gang Choi | Luke Lambourne | Michael A. Calderwood | Frederick P. Roth | Yun Shen | Joseph N. Paulson | Thomas Rolland | Francisco J. Campos-Laborie | Janusz Rak | Murat Taşan | Xinping Yang | Andrew MacWilliams | Amélie Dricot | Benoit Charloteaux | Omer Basha | Esti Yeger-Lotem | Dae-Kyum Kim | István A. Kovács | Robert J. Weatheritt | Katja Luck | Roujia Li | Miquel Duran-Frigola | Joseph C. Mellor | Marinella Gebbia | Carles Pons | Suzanne Gaudet | Adriana San-Miguel | Mohamed Helmy | Kerstin Spirohn | Irma Lemmens | Sudharshan Rangarajan | Wenting Bian | Javier De Las Rivas | Jean-Claude Twizere | Tong Hao | Yves Jacob | Nishka Kishore | Jan Tavernier | David E. Hill | Gloria M. Sheynkman | Carl Pollis | Sadie Schlabach | Suet-Feung Chin | Mariana Babor | Marc Vidal | Eyal Simonovsky | Bridget E. Begg | Ruth Brignall | Tiziana Cafarelli | Dongsic Choi | Atina G. Coté | Meaghan Daley | Steven Deimling | Alice Desbuleux | Madeleine F. Hardy | Jennifer J. Knapp | Miles W. Mee | Aaron D. Richardson | Bridget Teeking | Anupama Yadav | Dawit Balcha | Christian Bowman-Colin | Claudia Colabella | Georges Coppin | Cassandra D’Amata | David De Ridder | Steffi De Rouck | Hanane Ennajdaoui | Florian Goebels | Liana Goehring | Anjali Gopal | Ghazal Haddad | Elodie Hatchi | Yoseph Kassa | Serena Landini | Natascha van Lieshout | Dylan Markey | John Rasla | Ashyad Rayhan | Dayag Sheykhkarimli | Alexander Tejeda | Vincent Tropepe | Jochen Weile | Quan Zhong | M. Vidal | S. Gaudet | M. Calderwood | A. Dricot | M. Tasan | P. Aloy | B. Charloteaux | S. Chin | Yu Xia | M. Gebbia | I. Lemmens | J. Tavernier | J. Twizere | Q. Zhong | Y. Jacob | T. Rolland | Yun Shen | Katja Luck | Kerstin Spirohn | Yang Wang | Carl Pollis | Sadie Schlabach | Dae-Kyum Kim | Nishka Kishore | Xinping Yang | Dawit Balcha | M. Hardy | John Rasla | Alexander O. Tejeda | G. Sheynkman | Esti Yeger-Lotem | J. Paulson | Jochen Weile | Jennifer J. Knapp | J. Mellor | Carles Pons | N. van Lieshout | Miquel Duran-Frigola | Steven J. Deimling | V. Tropepe | L. Lambourne | R. Brignall | T. Cafarelli | F. Campos-Laborie | D. Choi | Meaghan C. Daley | A. Desbuleux | Aaron D. Richardson | Bridget Teeking | Anupama Yadav | Mariana Babor | C. Bowman-Colin | Soon-gang Choi | Claudia Colabella | Georges Coppin | Cassandra D’Amata | D. de Ridder | Steffi de Rouck | Hanane Ennajdaoui | Florian Goebels | Liana J Goehring | Anjali Gopal | Ghazal Haddad | Elodie Hatchi | M. Helmy | Yoseph Kassa | Serena Landini | Roujia Li | D. Markey | Ashyad Rayhan | A. San-Miguel | Dayag Sheykhkarimli | Eyal Simonovsky | Y. Wang | J. Rak | Yu-ting Xia | F. Roth | A. MacWilliams | J. de Las Rivas | D. Hill | Sudharshan Rangarajan | Marc Vidal | Jan Tavernier | Omer Basha | Tong Hao | Wenting Bian | J. Rak | J De Las Rivas | S. Choi | Dongsic Choi | Amélie Dricot | Ruth Brignall | Christian Bowman-Colin | Yun A Shen | D. Hill | Alice Desbuleux | Gloria M. Sheynkman

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