A novel pseudo-complementary PNA G-C base pair

Pseudo-complementary oligonucleotide analogues and mimics provide novel opportunities for targeting duplex structures in RNA and DNA. Previously, a pseudo-complementary A-T base pair has been introduced. Towards sequence unrestricted targeting, a pseudo-complementary G-C base pair consisting of the unnatural nucleobases N6-methoxy-2,6-diaminopurine (previously described in a DNA context), and N4-benzoylcytosine is now presented for design of pseudo-complementary PNA oligomers (pcPNAs).

[1]  M. Komiyama,et al.  Homologous recombination in human cells using artificial restriction DNA cutter. , 2009, Chemical communications.

[2]  M. Komiyama,et al.  Site-selective scission of human genome by artificial restriction DNA cutter. , 2009, Chemical communications.

[3]  Makoto Komiyama,et al.  Artificial Restriction DNA Cutters as New Tools for Gene Manipulation , 2009, Chembiochem : a European journal of chemical biology.

[4]  P. Glazer,et al.  Targeted correction of a thalassemia-associated β-globin mutation induced by pseudo-complementary peptide nucleic acids , 2009, Nucleic acids research.

[5]  M. Komiyama,et al.  Origin of high fidelity in target-sequence recognition by PNA-Ce(IV)/EDTA combinations as site-selective DNA cutters. , 2009, Journal of the American Chemical Society.

[6]  A. Lebedev,et al.  Properties of pseudo-complementary DNA substituted with weakly pairing analogs of guanine or cytosine , 2008, Nucleic acids research.

[7]  A. Lebedev,et al.  Enzymatic synthesis of structure-free DNA with pseudo-complementary properties , 2008, Nucleic acids research.

[8]  P. Glazer,et al.  Site-directed gene mutation at mixed sequence targets by psoralen-conjugated pseudo-complementary peptide nucleic acids , 2007, Nucleic acids research.

[9]  A. Gewirtz,et al.  Unrestricted accessibility of short oligonucleotides to RNA. , 2005, RNA.

[10]  Christoph Rosenbohm,et al.  Dry Column Vacuum Chromatography , 2004 .

[11]  Peter E Nielsen,et al.  Pseudocomplementary PNAs as selective modifiers of protein activity on duplex DNA: the case of type IIs restriction enzymes. , 2003, Nucleic acids research.

[12]  Peter E. Nielsen,et al.  Kinetics and mechanism of the DNA double helix invasion by pseudocomplementary peptide nucleic acids , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[13]  V V Demidov,et al.  Sequence-specific protection of duplex DNA against restriction and methylation enzymes by pseudocomplementary PNAs. , 2000, Biochemistry.

[14]  P. Nielsen,et al.  Double duplex invasion by peptide nucleic acid: a general principle for sequence-specific targeting of double-stranded DNA. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[15]  P. Nielsen,et al.  Inhibition of PNA triplex formation by N4-benzoylated cytosine. , 1998, Nucleic acids research.

[16]  R. Wiethe,et al.  Fmoc mediated synthesis of Peptide Nucleic Acids , 1995 .

[17]  R. H. Berg,et al.  Synthesis of Peptide Nucleic Acid Monomers Containing the Four Natural Nucleobases: Thymine, Cytosine, Adenine, and Guanine and Their Oligomerization , 1994 .

[18]  D. M. Brown,et al.  Synthesis and duplex stability of oligonucleotides containing adenine-guanine analogues. , 1992, Carbohydrate research.

[19]  R. Major,et al.  Additions and Corrections - Substituted O-Alkyl Hydroxylamines Chemically Related to Medicinally Valuable Amines , 1928 .

[20]  R. Major,et al.  SUBSTITUTED O-ALKYL HYDROXYLAMINES CHEMICALLY RELATED TO MEDICINALLY VALUABLE AMINES1 , 1927 .