Adsorption characteristics of oligopeptides composed of acidic and basic amino acids on titanium surface.

The adsorption characteristics of octapeptides, containing different numbers of aspartic acid, lysine, and alanine residues (i.e., D(4)K(0)A(4), D(4)K(1)A(3), D(4)K(3)A(1), D(4)K(4)A(0), and D(0)K(4)A(4)) on the surface of titanium (Ti) particles were investigated in the pH range of 3.0-8.8 at 30 degrees C. The adsorption isotherms for octapeptides having four plural aspartic acid residues with or without lysine residues showed two distinct adsorption modes, i.e., irreversible and reversible modes, at pHs 3.0-6.5; at pH 7.0 or higher, the adsorption mode was reversible. Increasing the number of lysine residues at a fixed number of aspartic acid residues (i.e., 4) decreased the amount of peptides adsorbed in both modes. D(4)K(4)A(0) adsorbed irreversibly at pHs 3.0-6.5, due to the fact that negatively charged carboxyl groups directly interact with a positively charged Ti surface, whereas positively charged amino groups of lysine residues are directed in an opposite direction toward the solution side, as predicted by molecular mechanics/dynamics calculations.

[1]  J. Ramsden.  Puzzles and paradoxes in protein adsorption , 1995 .

[2]  G. A. Parks,et al.  THE ZERO POINT OF CHARGE OF OXIDES1 , 1962 .

[3]  A. J. McQuillan,et al.  Monitoring Hydrous Metal Oxide Surface Charge and Adsorption by STIRS , 1997 .

[4]  K. Imamura,et al.  On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon. , 2001, Journal of bioscience and bioengineering.

[5]  S. Kittaka.  Isoelectric point of Al2O3, Cr2O3 and Fe2O3. I. Effect of heat treatment , 1974 .

[6]  G. Fields,et al.  Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. , 2009, International journal of peptide and protein research.

[7]  T. Arnebrant,et al.  Adsorption of α-lactalbumin and β-lactoglobulin on metal surfaces versus temperature , 1987 .

[8]  K. Imamura,et al.  Adsorption characteristics of various organic substances on the surfaces of tantalum, titanium, and zirconium. , 2005, Journal of colloid and interface science.

[9]  K. Imamura,et al.  Effects of carboxyl groups on the adsorption behavior of low-molecular-weight substances on a stainless steel surface. , 2004, Journal of colloid and interface science.

[10]  K. Nakanishi,et al.  Adsorption of Protein onto Stainless Steel Particle Surface and its Desorption Behavior , 1994 .

[11]  S. Kittaka.  Isoelectric point of Al2O3, Cr2O3 and Fe2O3. II. Binary solid solution , 1974 .

[12]  P. Cremer,et al.  Investigation of Water Structure at the TiO2/Aqueous Interface , 2004 .

[13]  George A. Parks,et al.  The Isoelectric Points of Solid Oxides, Solid Hydroxides, and Aqueous Hydroxo Complex Systems , 1965 .

[14]  K. Imamura,et al.  Adsorption characteristics of tryptic fragments of bovine beta-lactoglobulin on a stainless steel surface. , 1999, Journal of bioscience and bioengineering.

[15]  S. Ardizzone,et al.  Interfacial properties of oxides with technological impact in electrochemistry , 1996 .

[16]  U. Singh,et al.  A NEW FORCE FIELD FOR MOLECULAR MECHANICAL SIMULATION OF NUCLEIC ACIDS AND PROTEINS , 1984 .

[17]  Takaharu Sakiyama,et al.  Contribution of acidic amino residues to the adsorption of peptides onto a stainless steel surface. , 2003, Journal of colloid and interface science.

[18]  B. Kasemo,et al.  Implant Surfaces and Interface Processes , 1999, Advances in dental research.

[19]  McQuillan,et al.  An in Situ Infrared Spectroscopic Investigation of Lysine Peptide and Polylysine Adsorption to TiO(2) from Aqueous Solutions. , 1999, Journal of colloid and interface science.

[20]  P. Kollman,et al.  An all atom force field for simulations of proteins and nucleic acids , 1986, Journal of computational chemistry.

[21]  A. J. McQuillan,et al.  An In Situ Infrared Spectroscopic Study of the Adsorption of Lysine to TiO2 from an Aqueous Solution , 1998 .

[22]  K. Imamura,et al.  In Situ Analysis of the Removal Behavior of Protein Adsorbed on a Titanium Surface by H2O2-Electrolysis Treatment , 2002 .

[23]  K. Imamura,et al.  Protease susceptibility of beta-lactoglobulin adsorbed on stainless steel surface as evidence of contribution of its specific segment to adsorption. , 2006, Journal of bioscience and bioengineering.

[24]  K. Nakanishi,et al.  Adsorption of β-Lactoglobulin onto the Surface of Stainless Steel Particles , 1995 .

[25]  K. Imamura,et al.  Adsorption characteristics of bovine serum albumin and its peptide fragments on a stainless steel surface , 2004 .