How do inhibitors and modifiers of individual enzymes affect steady-state fluxes and concentrations in metabolic systems?

Abstract By using metabolic control theory we show how one can calculate the effect on steady-state metabolite concentrations and steady-state fluxes of small amounts of inhibitors or activators of individual enzymes in a metabolic system of arbitrary complexity. It turns out that only limited knowledge of the kinetics of the enzymes in the systems, summarized in their “Elasticity Coefficients,” is needed. An example of an actual calculation is given.

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