A syringe electrode device for simultaneous injection of DNA and electrotransfer.

Electroporation for gene delivery has attracted considerable attention recently, because of both the site-specific nature of the delivery and the high efficiency of the method. Electrotransfer of genes involves the application of an electric field to cells to enhance their permeability, facilitating exogenous polynucleotide transit across the cytoplasmic membrane. However, the relatively high electric field strength required for electroporation induces tissue damage, thus limiting its widespread application. We describe here a syringe electrode of our design, with which the same transfection efficiency can be achieved by using much lower electric field strength than that of conventional electrodes, such that the tissue damage is minimized.

[1]  A. Nishikawa,et al.  Visualization of dystrophic muscle fibers in mdx mouse by vital staining with Evans blue: evidence of apoptosis in dystrophin-deficient muscle. , 1995, Journal of biochemistry.

[2]  H. Aihara,et al.  Gene transfer into muscle by electroporation in vivo. , 1998, Nature biotechnology.

[3]  G. Repetto,et al.  Direct gene transfer into nonhuman primate myofibers in vivo. , 1992, Human gene therapy.

[4]  Jaroszeski,et al.  In vivo gene delivery by electroporation. , 1999, Advanced drug delivery reviews.

[5]  M. Jaroszeski,et al.  Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo , 2000, Gene Therapy.

[6]  D. Weiner,et al.  Modulating the immune response to genetic immunization , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[7]  Darius Moradpour,et al.  In vivo gene electroinjection and expression in rat liver , 1996, FEBS letters.

[8]  M J Jaroszeski,et al.  Theory and in vivo application of electroporative gene delivery. , 2000, Molecular therapy : the journal of the American Society of Gene Therapy.

[9]  T. Muramatsu,et al.  Foreign gene expression in the mouse testis by localized in vivo gene transfer. , 1997, Biochemical and biophysical research communications.

[10]  D Miklavcic,et al.  A validated model of in vivo electric field distribution in tissues for electrochemotherapy and for DNA electrotransfer for gene therapy. , 2000, Biochimica et biophysica acta.

[11]  Toshiyuki Matsuzaki,et al.  Direct gene transfer into rat liver cells by in vivo electroporation , 1998, FEBS letters.

[12]  M. Bureau,et al.  High-efficiency gene transfer into skeletal muscle mediated by electric pulses. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[13]  P Raskmark,et al.  In vivo electroporation of skeletal muscle: threshold, efficacy and relation to electric field distribution. , 1999, Biochimica et biophysica acta.

[14]  A. Nakamura,et al.  In vivo electroporation: a powerful and convenient means of nonviral gene transfer to tissues of living animals (Review). , 1998, International journal of molecular medicine.

[15]  M. Rols,et al.  In vivo electrically mediated protein and gene transfer in murine melanoma , 1998, Nature Biotechnology.

[16]  G. Ciliberto,et al.  Gene electrotransfer results in a high-level transduction of rat skeletal muscle and corrects anemia of renal failure. , 2000, Human gene therapy.