Assessment of amino acid requirements for optimum fermentation of xylan by mixed micro-organisms from the sheep rumen

A deletion approach was undertaken to identify which amino acids (AA) most limited the growth of mixed ruminal microorganisms on xylan. Ruminal fluid was withdrawn from sheep receiving a mixed grass hay/concentrate diet and incubated for 24 h with oat spelts xylan in the presence or absence of a mixture of 20 AA or the same mixture with a single AA deleted. Gas and volatile fatty acid production were increased by the AA mixture in comparison with incubations in which ammonia was the only added nitrogen (N) source, and the deletion of each of the aromatic AA, tyrosine, phenylalanine and tryptophan, as well as leucine and methionine, led to decreases ( P P P P > 0·05) influence on microbial yield, and the aromatic AA mixture also did not increase the microbial yield on xylan over the yield with ammonia as sole N source. It was concluded that aromatic AA may be first-limiting for xylan fermentation, but they cannot replace the benefits of a complete mixture of 20 AA in stimulating xylan fermentation by ruminal micro-organisms.

[1]  R. Wallace,et al.  Use of stable isotopes to measure de novo synthesis and turnover of amino acid-C and -N in mixed micro-organisms from the sheep rumen in vitro. , 2004, The British journal of nutrition.

[2]  V. Fievez,et al.  Is the synthesis of rumen bacterial protein limited by the availability of pre-formed amino acids and/or peptides? , 2004, The British journal of nutrition.

[3]  R. Wallace,et al.  Use of a deletion approach to assess the amino acid requirements for optimum fermentation by mixed micro-organisms from the sheep rumen , 2003 .

[4]  R. Onodera,et al.  Degradation of tryptophan and related indolic compounds by ruminal bacteria, protozoa and their mixture in vitro , 2003, Amino Acids.

[5]  M. Mitsumori,et al.  Stimulatory and inhibitory effects of protein amino acids on growth rate and efficiency of mixed ruminal bacteria. , 2002, Journal of dairy science.

[6]  R. Wallace,et al.  Incorporation of [15N]Ammonia by the Cellulolytic Ruminal Bacteria Fibrobacter succinogenesBL2, Ruminococcus albus SY3, and Ruminococcus flavefaciens 17 , 2001, Applied and Environmental Microbiology.

[7]  E. L. Miller,et al.  Effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in an in vitro semi-continuous culture system (RUSITEC) , 1999, British Journal of Nutrition.

[8]  R. Wallace,et al.  Influence of peptides and amino acids on fermentation rate and de novo synthesis of amino acids by mixed micro-organisms from the sheep rumen , 1999, British Journal of Nutrition.

[9]  Tuula T. Teeri,et al.  The roles and function of cellulose-binding domains , 1997 .

[10]  R. Onodera,et al.  Synthesis of phenylalanine and production of other related compounds from phenylpyruvic acid and phenylacetic acid by ruminal bacteria, protozoa, and their mixture in vitro. , 1997, The Journal of general and applied microbiology.

[11]  M. Cotta,et al.  Metabolism of nitrogen-containing compounds , 1997 .

[12]  C. Stewart,et al.  The Rumen Microbial Ecosystem , 1997, Springer Netherlands.

[13]  I. Armstead,et al.  The in vitro uptake and metabolism of peptides and amino acids by five species of rumen bacteria. , 1995, The Journal of applied bacteriology.

[14]  C. Stewart,et al.  Influence of peptides, amino acids and urea on microbial activity in the rumen of sheep receiving grass hay and on the growth of rumen bacteria in vitro , 1994 .

[15]  I. Armstead,et al.  Variations in the uptake and metabolism of peptides and amino acids by mixed ruminal bacteria in vitro , 1993, Applied and environmental microbiology.

[16]  P. V. Soest,et al.  A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. , 1992, Journal of animal science.

[17]  R. L. Baldwin,et al.  Effects of amino acids and peptides on rumen microbial growth yields. , 1989, Journal of dairy science.

[18]  K. Moore,et al.  Disappearance of hemicellulosic monosaccharides and alkali-soluble phenolic compounds of normal and brown midrib sorghum x sudangrasses fed to heifers and sheep. , 1989, Journal of dairy science.

[19]  Kh Menke,et al.  Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid , 1988 .

[20]  M. Cotta,et al.  Effect of 3-Phenylpropanoic Acid on Growth of and Cellulose Utilization by Cellulolytic Ruminal Bacteria , 1986, Applied and environmental microbiology.

[21]  S. Duncan,et al.  The effect of avoparcin on cellulolytic bacteria of the ovine rumen , 1985 .

[22]  J. Nolan,et al.  Nitrogen metabolism in the rumen. , 1984, Journal of dairy science.

[23]  M. Cotta,et al.  Effect of Peptides and Amino Acids on Efficiency of Rumen Bacterial Protein Synthesis in Continuous Culture , 1982 .

[24]  R. Wallace.  Effect of ammonia concentration on the composition, hydrolytic activity and nitrogen metabolism of the microbial flora of the rumen. , 1979, The Journal of applied bacteriology.

[25]  J. Nolan,et al.  Fermentation and nitrogen dynamics in Merino sheep given a low-quality-roughage diet , 1979, British Journal of Nutrition.

[26]  N. Russell,et al.  Degradation of amino acids by pure cultures of rumen bacteria. , 1976, Journal of animal science.

[27]  W. Chalupa.  Degradation of amino acids by the mixed rumen microbial population. , 1976, Journal of animal science.

[28]  J. Nolan,et al.  Further studies of the dynamics of nitrogen metabolism in sheep , 1976, British Journal of Nutrition.

[29]  C. V. Van Nevel,et al.  Rumen microbial growth rates and yields: effect of amino acids and protein. , 1976, Journal of dairy science.

[30]  F. Sauer,et al.  Amino acid biosynthesis in mixed rumen cultures. , 1975, The Biochemical journal.

[31]  S. Kristensen.  Ruminal bioshynthesis of aromatic amino acids from arylacetic acids, glucose, shikimic acid and phenol , 1974, British Journal of Nutrition.

[32]  M. P. Bryant.  Nutritional requirements of the predominant rumen cellulolytic bacteria. , 1973, Federation proceedings.

[33]  M. Allison.  Biosynthesis of amono acids by ruminal microorganisms. , 1969, Journal of animal science.

[34]  J. Leibholz.  Effect of Diet on the Concentration of Free Amino Acids, Ammonia and Urea in the Rumen Liquor and Blood Plasma of the Sheep 1,2 , 1969 .

[35]  M. P. Bryant,et al.  Oligopeptide Uptake by Bacteroides ruminicola , 1967, Journal of bacteriology.

[36]  A. Virtanen.  Milk Production of Cows on Protein-Free Feed , 1966, Science.

[37]  T. W. Scott,et al.  The formation and metabolism of phenyl-substituted fatty acids in the ruminant. , 1964, The Biochemical journal.

[38]  M. P. Bryant,et al.  SOME NUTRITIONAL CHARACTERISTICS OF PREDOMINANT CULTURABLE RUMINAL BACTERIA , 1962, Journal of bacteriology.

[39]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .