Robust SFC method optimization using design space strategy

Recently, the number of publications about SFC knew an important increase. This phenomenon could be explained by the necessity of rapid, effective and green analytical methods. In order to prove the potential of SFC, the aim of our work was to develop SFC methods for the separation of pharmaceutical compounds using an innovative chemometric approach. Two case studies were investigated in the present project; first, polar compounds were selected as a worst case; secondly, the method development and validation of antibiotic drugs were selected as a more practical case.