New Rice Seedling Test for Gibberellins―Microdrop Method

The gibberellins were originally discovered by Japanese scientists as metabolic products of a fungus, Gibberella fujilcuroi. They have a considerable effect to promote the growth of intact plants. In addition, the substances which are obtained from other sources and have s imilar effect have also been called gibberellins. The symbols A1, A,, etc. have been used to denote the characterized gibberellins. > Gibberellin-like substances are now known to be widely distributed in higher plants, and thus appear to be important in controlling many different phases of plant growth and development. Many workers have provided various bioassays fo r detecting g·ibberellins in plant tissues. Seedli ngs of normal Oryza sativa,3> dwarf mutants of Zea mays > and dwarf cu ltivars of Pismn sativuml) have been used as the assay organism. The present writer•>.» devised a rapid and simple bioassay for gibberellins which was based upon the elongation response of the second leaf sheath of the dwarf cult ivars of Oryza sativa to gibberellins. Evidence for the occurrence of bamboo gibberellin (GA,o) in r ice shoots will be reported as an example of the use of this method.