In vitro assay of erythropoietin: simple determination in a small amount of human serum samples.

An in vitro method for the determination of erythropoietin (EPO) titres in human serum samples was developed to detect low levels of EPO titres in various haematological states. Fetal mouse liver cells (FMLC) were incubated in methylcellulose-containing media, and radioiron incorporation into haem was estimated in cultured FMLC. We could determine the concentrations of EPO from around 20 to 600 mU per ml of a very small amount of serum samples from patients and normal adults. Using this method, EPO titres of normal adults (54 +/- 31 mU/ml), chronic renal failure patients (29 +/- 23 mU/ml), aplastic anaemia patients (545 +/- 74 mU/ml), and polycythaemia vera patients (16 +/- 7 mU/ml) were determined.

[1]  F. Takaku,et al.  Response of uraemic bone marrow cells to erythropoietin in vitro. , 2009, Scandinavian journal of haematology.

[2]  J. Fisher,et al.  Identification of spermine as an inhibitor of erythropoiesis in patients with chronic renal failure. , 1981, The Journal of clinical investigation.

[3]  J. Espada,et al.  In Vitro Assay of Erythropoietin in Fetal Mouse: Liver Cultures , 1981, British journal of haematology.

[4]  N. Iscove,et al.  Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol. , 1980, Experimental cell research.

[5]  P. Hågå,et al.  In vitro assay for erythropoietin: erythroid colony formation in methyl cellulose used for the measurement of erythropoietin in plasma , 1979 .

[6]  J. Fisher,et al.  Inhibitors of erythroid colony-forming cells (CFU-E and BFU-E) in sera of azotemic patients with anemia of renal disease. , 1978, The Journal of laboratory and clinical medicine.

[7]  A. Hart,et al.  Modified method of erythropoietin (ESF) bioassay in vitro using mouse fetal liver cells. II. measurement of ESF in human serum. , 1978, Blood.

[8]  A. Hart,et al.  Modified method of erythropoietin (ESF) bioassay in vitro using mouse fetal liver cells. I. Effect of serum iron on 59Fe incorporation into heme. , 1978, Blood.

[9]  R. Parmley,et al.  Human marrow erythropoiesis in culture. I. Characterization of methylcellulose colony assay. , 1976, Blood.

[10]  A. Alfrey,et al.  The anemia of chronic renal failure and chronic diseases: in vitro studies of erythropoiesis. , 1976, Blood.

[11]  E. McCulloch,et al.  Erythropietic colonies in cultures of human marrow. , 1974, Blood.

[12]  F. Sieber,et al.  Erythroid colony formation in cultures of mouse and human bone marrow: Analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose‐concanavalin A , 1974, Journal of cellular physiology.

[13]  T. McDonald,et al.  The Hemagglutination-Inhibition Assay of Erythropoietin , 1971 .

[14]  J. P. Chen,et al.  Erythropoietin assays: some new and different approaches. , 1980, Experimental hematology.

[15]  P. Hågå,et al.  In vitro assay for erythropoietin: erythroid colony formation in methyl cellulose used for the measurement of erythropoietin in plasma. , 1979, Blood.

[16]  F. Sieber,et al.  Erythroid progenitors in mouse bone marrow detected by macroscopic colony formation in culture. , 1975, Experimental hematology.

[17]  Dunn Cd,et al.  A quantitative bioassay for erythropoietin using mouse fetal liver cells. , 1975 .

[18]  C. D. Dunn,et al.  A quantitative bioassay for erythropoietin using mouse fetal liver cells. , 1975, Experimental hematology.

[19]  F. Teale,et al.  Cleavage of the haem-protein link by acid methylethylketone. , 1959, Biochimica et biophysica acta.