ANTARCTIC DISTRIBUTION, PIGMENT AND LIPID COMPOSITION, AND MOLECULAR IDENTIFICATION OF THE BRINE DINOFLAGELLATE POLARELLA GLACIALIS (DINOPHYCEAE) 1

Polarella glacialis (Montresor et al.) was identified in Davis Station sea ice by morphological and DNA sequence comparison of cultures with those of the authentic strain P. glacialis CCMP 1383 isolated from McMurdo Sound. Cells and cysts of the Davis isolate (FL1B) were morphologically indistinguishable from P. glacialis, and comparison of the large subunit rDNA of both cultures demonstrated only 0.2% sequence divergence over 1366 base pairs. The photosynthetic pigments of P. glacialis (strains FL1B and CCMP 1383) were typical of dinoflagellates, with peridinin (contributing up to 31%) as the major accessory pigment. Extremely high levels of polyunsaturated fatty acids (PUFA, up to 76.3%) were characteristic of P. glacialis isolate FL1B. The high PUFA concentration of this species is thought to be an adaptation to survive the cold temperatures of the upper fast ice. The sterol profile of FL1B was atypical of dinoflagellates, with 4‐desmethylsterols (up to 79%) in greater abundance than 4α‐methyl sterols (up to 24%). 27‐Nor‐24‐methylcholest‐5,22E‐dien‐3β‐ol was identified as the principle sterol in P. glacialis, contributing up to 64% of the total sterol composition.

[1]  S. Blackburn,et al.  Very-long-chain (C28) highly unsaturated fatty acids in marine dinoflagellates , 1999 .

[2]  Geoffrey Eglinton,et al.  Lipid composition of the marine dinoflagellate Scrippsiella trochoidea , 1988 .

[3]  S. Wright,et al.  Qualitative and quantitative HPLC analysis of SCOR reference algal cultures , 1997 .

[4]  S. M. Barrett,et al.  Changes in the lipid composition and maximisation of the polyunsaturated fatty acid content of three microalgae grown in mass culture , 1993, Journal of Applied Phycology.

[5]  K. Buck,et al.  A DINOFLAGELLATE CYST FROM ANTARCTIC SEA ICE 1 , 1992 .

[6]  P. Nichols,et al.  Applications of thin layer chromatography flame ionization detection to the analysis of lipids and pollutants in marine and environmental samples , 1991 .

[7]  S. M. Barrett,et al.  Microalgal biomarkers: A review of recent research developments , 1998 .

[8]  Graeme A. Dunstan,et al.  Biochemical composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 2. Lipid classes and fatty acids , 1992 .

[9]  Y. Ishida,et al.  ANALYSIS OF ALEXANDRIUM (DINOPHYCEAE) SPECIES USING SEQUENCES OF THE 5.8S RIBOSOMAL DNA AND INTERNAL TRANSCRIBED SPACER REGIONS 1 , 1996 .

[10]  R. B. Johns,et al.  The fatty acid and sterol composition of two marine dinoflagellates , 1984 .

[11]  C. Lovejoy,et al.  Bipolar distribution of the cyst-forming dinoflagellate Polarella glacialis , 2003, Polar Biology.

[12]  K. Buck,et al.  Changes in the sea-ice brine community during the spring-summer transition , McMurdo Sound , Antarctica . I . Photosynthetic protists , 2006 .

[13]  G. Hallegraeff,et al.  Gymnodinium microreticulatum sp. nov. (Dinophyceae): a naked, microreticulate cyst-producing dinoflagellate, distinct from Gymnodinium catenatum and Gymnodinium nolleri , 1999 .

[14]  D. Stoecker,et al.  POPULATION DYNAMICS OF MICROALGAE IN THE UPPER LAND‐FAST SEA ICE AT A SNOW‐FREE LOCATION , 1998 .

[15]  D. Stoecker,et al.  EXCYSTMENT AND GROWTH OF CHRYSOPHYTES AND DINOFLAGELLATES AT LOW TEMPERATURES AND HIGH SALINITIES IN ANTARCTIC SEA‐ICE 1 , 1997 .

[16]  D. Hodgson,et al.  Summer phytoplankton succession in Ellis Fjord, eastern Antarctica , 1993 .

[17]  H. Harvey,et al.  Lipid Composition of the Marine Ciliates Pleuronema sp. and Fabrea salina: Shifts in Response to Changes in Diet 1 , 1997 .

[18]  D. Anderson,et al.  IDENTIFICATION OF GROUP‐ AND STRAIN‐SPECIFIC GENETIC MARKERS FOR GLOBALLY DISTRIBUTED ALEXANDRIUM (DINOPHYCEAE). II. SEQUENCE ANALYSIS OF A FRAGMENT OF THE LSU rRNA GENE 1 , 1994 .

[19]  S. Liaaen-Jensen,et al.  Carotenoids of the dinophyceae , 1974 .

[20]  H. Harvey,et al.  Marine ciliates as a widespread source of tetrahymanol and hopan-3β-ol in sediments , 1991 .

[21]  S. W. Wrightl.  Analysis of phytoplankton of the Australian sector of the Southern Ocean : comparisons of microscopy and size frequency data with interpretations of pigment HPLC data using the ' CHEMTAX ' matrix factorisation program , 2006 .

[22]  S. Wright,et al.  Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods , 1997 .

[23]  G. Napolitano,et al.  All-cis-3,6,9,12,15-octadecapentaenoic acid: A problem of resolution in the GC analysis of marine fatty acids , 1988 .

[24]  Ø. Moestrup,et al.  Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmoured dinoflagellates , 2000 .

[25]  S. Wakeham.  Lipid biomarkers for heterotrophic alteration of suspended particulate organic matter in oxygenated and anoxic water columns of the ocean , 1995 .

[26]  Peter D. Nichols,et al.  PIGMENTS, FATTY ACIDS, AND STEROLS OF THE TOXIC DINOFLAGELLATE GYMNODINIUM CATENATUM 1 , 1991 .

[27]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[28]  S. Blackburn,et al.  THE FATTY ACID AND STEROL COMPOSITION OF FIVE MARINE DINOFLAGELLATES , 1999 .

[29]  J. Dick,et al.  Octadecapentaenoic acid in a raphidophyte alga Heterosigma akashiwo , 1997 .

[30]  T. McMeekin,et al.  Lipids of cultured Phaeocystis pouchetii: Signatures for food-web, biogeochemical and environmental studies in Antarctica and the Southern ocean , 1991 .

[31]  Jeffrey D. Leblond,et al.  A SURVEY OF THE STEROL COMPOSITION OF THE MARINE DINOFLAGELLATES KARENIA BREVIS, KARENIA MIKIMOTOI, AND KARLODINIUM MICRUM: DISTRIBUTION OF STEROLS WITHIN OTHER MEMBERS OF THE CLASS DINOPHYCEAE1 , 2002 .

[32]  S. Wakeham,et al.  Fatty acid and sterol biomarkers as indicators of particulate matter source and alteration processes in the Black Sea , 1991 .

[33]  J. Garrido,et al.  Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases , 2000 .

[34]  K. Buck,et al.  The biota of Antarctic pack ice in the Weddell sea and Antarctic Peninsula regions , 1989, Polar Biology.

[35]  L. Medlin,et al.  Genetic differentiation among three colony-forming species of Phaeocystis: further evidence for the phylogeny of the Prymnesiophyta , 1994 .

[36]  S. W. Jeffrey,et al.  CHLOROPLAST PIGMENT PATTERNS IN DINOFLAGELLATES 1 , 1975 .

[37]  R. Pistocchi,et al.  INVESTIGATION OF 4‐METHYL STEROLS FROM CULTURED DINOFLAGELLATE ALGAL STRAINS , 1997 .

[38]  D. Nichols,et al.  Fatty acid, sterol and hydrocarbon composition of Antarctic sea ice diatom communities during the spring bloom in McMurdo Sound , 1993, Antarctic Science.

[39]  G. Procaccini,et al.  POLARELLA GLACIALIS, GEN. NOV., SP. NOV. (DINOPHYCEAE): SUESSIACEAE ARE STILL ALIVE! , 1999 .

[40]  S. Wright,et al.  Analysis of phytoplankton of the Australian sector of the Southern Ocean: comparisons of microscopy and size frequency data with interpretations of pigment HPLC data using the \'CHEMTAX\' matrix factorisation program , 1996 .

[41]  D. Repeta,et al.  Calibration methods for HPLC , 1997 .

[42]  P. Nichols,et al.  Fatty acid and lipid composition of 10 species of microalgae used in mariculture , 1989 .

[43]  J. Harwood,et al.  Lipids in plants and microbes , 1984 .

[44]  Heterogeneity of SSU and LSU rDNA sequences of Alexandrium species , 1998 .