Sensitivity of Actual Laboratory Diagnostic Methods Used for Surveillance of Swine brucellosis in Croatia S

During three years (2009-2011) 240 organ and tissue samples from swine serologically positive on brucellosis were bacteriologically tested for brucellosis. The samples were obtained from country farms from seven counties and two wild boars organs were obtained from hunting ground. Brucella was isolated from 52 (21.5%) samples. Infection with Brucella suis bv. 2 was confirmed in 12 (29.3%) swine herds, and including the positive tests of wild boars, infection was confirmed in 5 counties. According to consecutive negative bacteriological testing in flocks and epidemiological investigation we also confirmed false positive serological reactions (FPSRs) in 29 seropositive herds (70.7%). Bacteriological investigation in future should be improved by using new selective media, but more imporant, better sampling management and prompt sample delivery should be achieved to provide higher method sensitivity. Detection of brucellosis in wild boars over years confirms permanent source of disease for free range swine in all parts of Croatia.

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